摘要
建株的大鼠肾上腺嗜铬细胞瘤ＰＣ１２细胞，在神经生长因子ＮＧＦ的作用下，会长出神经突起．这一现象被用于神经生长因子的活性测定．但就定量测定而言，目前已报道的方法、方案、包括判定阳性细胞的标准，均有一定差异，而我国也尚未建立起对这一重要分子活性的标准测定法．通过比较两种不同的方案，提示高浓度的神经生长因子先行对细胞进行长时间的预处理并非为测定所必需；同时提出了比较简便可行的准则，包括判定阳性细胞的标准，对方法的不足也进行了讨论．
Cultured rat pheochromocytoma PC12 cells, exposed to nerve growth factor (NGF), can form neurite processes. This phenomenon has been used for NGF bioassay. However the protocols reported for quantitative assay of NGF, including the criteria for judging positive cells, are various. In China the standard method for measuring this important molecule has not been established yet. In this study, two different protocals were compared, suggesting that long-period pretreatment of PC12 cells with concentrated NGF was not of prerequisite for quantitative assay of NGF activity. This simple and easily-performed protocal was recommended and its shortage was discussed.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期218-220,共3页
Journal of Fudan University：Natural Science
基金
烟台荣昌制药有限公司资助课题
