摘要
以实验室自行克隆的新基因ＺＮＦ１９１顺序为材料，按三联体密码转换出锌指基因编 码的４个锌指结构域ＺＮＦ１９１ａ，ｂ，ｃ和ｄ的蛋白质一级顺序，并将它们与已用晶体衍射法确 证了空间构象的７个Ｃｙｓ２Ｈｉｓ２（Ｃ２Ｈ２）型锌指进行同源比较，从中发现锌指７ＺＮＦ和１ＺＮＦ的 一级结构与待检验的锌指顺序同源度高且间隙较小．然后，运用 ＱＵＡＮＴＡ软件包对 ４个锌 指结构区 ＺＮＦ１９１ａ，ｂ，ｃ和ｄ分别进行了计算机三维结构模拟，并以３Ｄ ｐｒｏｆｉｌｅ方法检验了该 结构的准确程度．分析结果提示：ＺＮＦ１９１基因的４个潜在锌指结构区均能够形成正常锌指 结构．
Three dimension conformation of four putative zinc-finger motifs coded by a novel gene ZNF191 cloned in our laboratory was constructed and examined by using the homologybased modeling system. Data of 15 known zinc-finger motifs, 7 of which are C2H2 type,in PDB protein database were selected and used to compare with linear sequence of the zinc-finger motifs to be tested. An alignment was made between the sequence of four putative zinc-finger motifes and that of seven C2H2-type motifs seperately. Using QUANTA software package, each con formation relating to putative zinc-finger motifs was constructed and then examined by 3D profile method. It is shown that all of the putative zinc-finger motifs can form a zincfinger conformation properly.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期123-128,共6页
Journal of Fudan University：Natural Science
基金
国家自然科学基金
上海市科技发展基金
