摘要
以2'-氯乙基二乙胺盐酸盐（DEAE）为载体修饰剂，对β-硫酸酯乙砜基苯胺（SESA）为载体活化剂，制备多孔球状碱性氨基苯磺酰乙基接枝淀粉（ABSE接枝淀粉）载体，采用重氮化方法固定化葡萄糖异构酶．测定DEAE基团量对固定化葡萄糖异构酶pH-活力的影响，同时对葡萄糖异构酶的最佳固定化条件和性质进行详细的研究．结果表明，DEAE基因虽然并没有使固定化异构酶的最适pH发生移动，但却使其加宽，于pH6仍保持最大活力85％．葡萄糖异构酶经固定化后pH稳定性明显提高，但是温度稳定性却有所下降．该固定化葡萄糖异构酶于4℃储存2个月，活力没有明显改变．
Glucose isomerase was immobilized onto DEAE-(4-amino-benzene sulfonylethyl-porous starch graft copolymers) beads by diazotization. Although the DEAE content did't make the optimum PH of IGI shift, it broadened the pH-activity curve of IGI. The IGI showed improved PH stability as compared to that of free enzyme. When stored at 4℃ for 2 months, no obvious change of IGI activity was found.
出处
《吉林大学自然科学学报》
CAS
CSCD
1998年第2期66-68,共3页
Acta Scientiarum Naturalium Universitatis Jilinensis
关键词
碱性
ABSE
接枝淀粉
固定化
葡萄糖异构酶
basic ABSE-starch graft copolymer beads, immobilization of glucose isomerase, diazotization
