摘要
以质粒PBR322为载体、BamHI部分酶切片段(4~10kb)为目的片段成功构建了大鼠基因组文库并进行了鉴定,文库总重组子数为3.2×107,插入比例95%,为大鼠APKD基因的克隆筛选以及有关大鼠基因的其它一些研究奠定了基础.
The rat genome library which has PBR322 as vector and 4~10 kb DNA segments cut by BamHI restriction enzyme as aim segments has been successfully constructed and identified. This library has 3.2×10 7 recombinants and the insertion ratio is 95%, which is the foundation of rat APKD gene clone selection and some other research work obout rat gene.
出处
《西南民族学院学报(自然科学版)》
CAS
1998年第1期42-47,共6页
Journal of Southwest Nationalities College(Natural Science Edition)