摘要
通过设计特异引物PR1/PR2和ST1/ST2分别扩增出香蕉枯萎病菌1号和4号生理小种的DNA特异片段,优化检测体系的反应参数,建立了香蕉枯萎病菌生理小种双重PCR检测方法,检测体系的最佳退火温度为56℃,检测灵敏度的最低起始DNA为200pg。应用结果表明该体系有较高的准确性和稳定性,在1次PCR扩增中引物PR1/PR2和ST1/ST2能同时检测香蕉枯萎病菌1号和4号生理小种。
Two paires of primers PR1/PR2 and ST1/ST2 were developed for rapid and accurate detection of race 1 and race 4 of Fusarium oxysporum f.sp.cubense （Foe）. The primers PR1/PR2 and ST1/ST2 could amplified specific DNA fragments for race 1 and race 4 of Foe. At the same time, the parameters of duplex PCR were optimized and the PCR sensitivity were tested and the duplex PCR assay for detection of physiological races of Foc was develped. The optimized conditions were an annealing at 56 ℃ The minimum amount of DNA of Foc could be detected was as low as 200 pg. It demonstrates that the duplex PCR approach will provided an accurate and stability method for detection of physiological races of Foe. The detection of racel and race 4 of Foc can be finished in a PCR amplification.
出处
《中国农学通报》
CSCD
北大核心
2009年第1期237-240,共4页
Chinese Agricultural Science Bulletin
基金
福建省重大科技专项"福建主要外来有害生物防控技术体系的研究"(2006NZ002)
农业部财政专项"香蕉枯萎病快速鉴定
监测
防治技术研究与药剂筛选"(2130108)
关键词
香蕉枯萎病菌
分子检测
双重PCR
Fusarium oxysporum f.sp. cubense, molecular detection, duplex PCR
