摘要
目的：研究类ＮＩＤＤＭ大鼠模型肝糖原合成酶的活性。方法：雄性Ｗｉｓｔａｒ大鼠４０只，随机分为两组，每组２０只，一组用小剂量链脲佐菌素（ＳＴＺ，３０ｍｇ／ｋｇ）尾静脉注射，另一组不给ＳＴＺ，作为对照组，观察１８周，选择糖耐量异常、胰岛素水平高于或等于对照组者作为类ＮＩＤＤＭ大鼠模型，实验末期测定其肝糖原合成酶的活性。结果：Ⅰ型酶为（０．１８±０．０６）μｍｏｌ／（ｍｉｎ·ｇ）相对于（０．２４±０．０９）μｍｏｌ／（ｍｉｎ·ｇ）（Ｐ＜０．０５），而总酶Ｉ＋Ｄ为（１．５±０．４）μｍｏｌ／（ｍｉｎ·ｇ）相对于（１．８±０．４）μｍｏｌ／（ｍｉｎ·ｇ）（Ｐ＜０．０５）。结论：糖尿病鼠此酶活性明显低于对照组。
Objective: To study the activity of liver glycogen synthase in analogous model of NIDDM rats. Methods: 40 male Wistar rats were divided into two groups at random. Each group consisted of 20 rats. The rats of one group were injected with low dose of streptozotocin(STZ)(30 mg/kg)via tail vena. The rats of the other group used as control were not injected with STZ. The rats among the group were injected with STZ whose glucose tolerance was impaired and the level of insulin equal to or higher than that of the control. After 18 weeks they were taken as the analogous rat model of NIDDM. The activity of liver glycogen synthase (GS)was assayed at the end of the experiment. Results: Type I enzyme: (0.18±0.06) μmol/(min·g )versus (0.24±0.09) μmol/(min·g) (P<0.05), and totalenzyme: (1.5±0 .4) μmol/(min·g)versus (1.8±0.4) μmol/(min·g) (P<0.05). Conclusion: The GS activity of diabetic rats was significantly lower than that of the control.
出处
《北京医科大学学报》
CSCD
1998年第1期46-48,共3页
Journal of Peking University(Health Sciences)
基金
国家教委回国人员启动基金
关键词
糖尿病
肝糖原合成酶
测定
NIDDM
Diabetes mellitus, Non-insulindependent/enzymol Glycogen synthase/metab Liver glycogen/biosyn Insulin resistance
