摘要
目的探讨严重急性呼吸综合征相关的冠状病毒（SARS-CoV）感染后炎症细胞因子的mRNA表达和分泌水平的动态变化，在蛋白质水平上阐明SARS-CoV的致病机理。方法本研究在建立树突状细胞（dendritic cells，DC）培养方法的前提下，利用SARS-CoV的N基因重组腺病毒（rAd-N）和对照的腺病毒（rAd-LacZ）来感染成熟的DC，用RT-PCR和ELISA法检测Dc对IL-1β、IL-6、IL-8和TNF-α的mRNA表达和分泌水平的变化。结果IL-6和TNF-α mRNA的表达和分泌水平在前24h之内是逐渐升高的，与对照组（rAd-LacZ感染）相比，差异有统计学意义（P〈0．05或P〈0．01）。结论SARS-CoV的N蛋白与SARS的急性期DC分泌过量的前炎症细胞因子有关。
Objective To investigate the exact mechanism of SARS-CoV pathogenesis at the protein level. Method Under the condition of the establishment of dendrtic ceils （DC） culture method, we used recombinant adenovirus to infect mature DC to make clear the development changes in mRNA levels and secreted protein levels of proinflammatory cytokines of IL-1β, IL-6, IL-8 and TNF-α by using RT-PCR and ELISA. Result We found that mRNA levels and secreted protein levels of IL-6 and TNF-α in DC had increased gradually after rAd- N infection during first 24 h compared with the control DC infected by rAd-LacZ（ P 〈 0.05 or P 〈 0.01 ）. Conclusion It is suggested that N protein may be related to the excessive secretion of proinflammatory cytokines during SARS-CoV infection at the acute phase.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第6期431-433,共3页
Chinese Journal of Experimental and Clinical Virology
基金
江苏省教育厅SARS专项课题资助项目（JH03-053）
