摘要
目的探讨胰岛素样生长因子结合蛋白-3(IGFBP-3)、视网膜X受体α(RXRα)及信号转导子和转录激动子(STAT-1)在β-淀粉样肽1～42(Aβ1～42)诱导大鼠海马神经元凋亡中的可能作用。方法以原代培养的大鼠海马神经元为模型,分为对照组和Aβ1～42组,加入不同浓度的凝聚态Aβ1～42,原位缺口末端标记法(TUNEL)观察凋亡细胞的形态;免疫细胞荧光方法检测凋亡细胞及IGFBP3、RXRα阳性细胞;免疫印迹法检测RXRα蛋白和STAT-1蛋白的表达水平。结果20μmol/LAβ1～42作用大鼠海马神经元24h能明显诱导神经元凋亡,表现为TUNEL/DAPI染色出现阳性细胞;20μmol/LAβ1～42作用大鼠海马神经元3～6h后IGFBP3阳性细胞、RXRα阳性细胞、RXRα蛋白的表达水平均较对照组有明显增高(P<0.01),在较高水平保持一段时间后逐渐下降;而STAT-1蛋白的表达水平则显著降低(P<0.01)。结论IGFBP3/RXRα或STAT-1信号转导通路可能在Aβ1～42诱导大鼠海马神经元凋亡中起一定作用。
Objective To investigate the effects of IGFBP-3, RXRα and STAT-1 on Aβ1-42 hippocampus neurons. Methods Apoptosis was induced by fibrillar Aβ1-42 . The percentage of neurons microscopy after staining with TUNEL/DAPI. IGFBP-3 and RXRa positive neurons were observed by induced apeptosis in rat apoptosis was evaluated by immunofluorescence. The expression of RXRa and STAT-1 protein were detected by Western blotting. Results After treatment with 20μmol/L Aβ1-42 for 24 hours, the apoptotic hippocampus neurons were shown by TUNEL/DAPI assay. The percentage of apoptotic neurons was increased in a time-dependent manner. During the development of apoptosis, both the percentage of IGFBP-3/ RXRa positive neurons and the expression of RXRa protein increased markedly after 3-6hours （P 〈 0.01）. However, the expression of STAT-1 protein significantly decreased （ P 〈 0.01 ）. Conclusion IGFBP-3/RXRa or STAT-1 signaling pathway may be a possible signaling pathway during Aβ1-42 induced apoptosis in rat hippocampus neurons.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第6期810-815,共6页
Acta Anatomica Sinica
基金
福建省自然科学基金资助项目(C0440010)
