期刊文献+

甘蔗叶汽爆液中挥发性微生物生长抑制物的鉴定 被引量:3

Identification of Volatile Inhibitors in Sugar Cane Leaf Hydrolysate by Steam-explosion
在线阅读 下载PDF
导出
摘要 通过气质联用仪(GC-MS)对甘蔗叶汽爆液的挥发性物质进行了分析,共鉴定出12个成分,有机酸占总挥发物含量的95%以上,丁酸、丙烯酸、巴豆酸、2-吡咯甲醛首次发现存在于爆破液中。分别考察了已鉴定成分在不同pH条件下对酿酒酵母(Saccharomyces cerevisiae)细胞生长的影响,结果表明,所有挥发成分在自然pH条件下均对酵母细胞生长产生明显的抑制作用,提高pH值能有效降低四碳以下有机酸对酵母细胞生长的毒害作用,但不能明显降低六碳有机酸及醛类化合物的毒性。 Volatile compounds in sugar cane leaf hydrolysate by steam-explosion were analyzed using GCMS. 12 compounds were identified. It was found that the organic acid accounted for more than 95% of the total volatile compounds. In these compositions, Butanoic acid, 2-Propenoic acid, Crotonic acid and 1H-Pyrrole-2-carboxaldehyde were first found in the hydrolysate by steam-explosion. The identified compounds were respectively added into the medium under different PH values so we could study their impacts on the growth of saccharomyces cerevisiae. The results indicated that all the volatile compounds inhibited the cell growth without adjusting pH value. When we raised the medium PH value to 7 the inhibitory toxicity of four-carbon organic acids were much lower, whereas there were no obvious reduction on the inhibitory toxicity of hexadcarbon organic acids and aldehydes.
出处 《食品与发酵工业》 CAS CSCD 北大核心 2008年第11期29-32,共4页 Food and Fermentation Industries
基金 国家自然科学基金(No.20666002) 广西自然科学基金(No.0731033)资助项目
关键词 甘蔗叶 蒸汽爆破 抑制物 气质联用 sugar cane leaf, steam-explosion, inhibitors, GC-MS
  • 相关文献

参考文献11

  • 1Ballesteros I, Negro M J, Oliva J M, et al. Ethanol Production from Steam-Explosion Pretreated Wheat Straw [J]. Applied Biochemistry and Biotechnology, 2006,130 ( 1-30) : 496-508
  • 2Shao Z, Tian Y, Tan H. Pretreatment of Corn Stalk by Steam Explosion [J]. Beiiing Institute of Technology, 2003,12(1) :85-88
  • 3Agblevorf A, Fu J, Haines B, et al. Identification of microbial inhibitory functional groups in corn stover hydrolysate by carbon-13 nuclear magnetic resonance spectrosco py [J]. Appl Biochem Biotehnol, 2004, 119(2) : 97-120
  • 4庄新姝,王树荣,袁振宏,骆仲泱,吴创之,岑可法.纤维素超低酸水解产物的分析[J].农业工程学报,2007,23(2):177-182. 被引量:40
  • 5Luo C,Brink D L,Blanch H W. Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanol[J]. Biomass and Bioenergy , 2002, 64(22) : 125-138
  • 6Larsson S,Palmqvist E, Hagerdal B,et al. The generation of fermentation inhibitors during dilute acid hydrolysis of softwood [J]. Enzyme and Microbial Technology, 1999, 24(3-4) :151-159
  • 7姚嘉旻,姜岷,陈可泉,申宁,苏溧,孙娜亚.改进DNS法测定玉米皮水解液中总糖含量[J].食品科技,2007,32(11):165-168. 被引量:10
  • 8张厚瑞,何成新,梁小燕,曾健智,唐峰.半纤维素水解物生物转化生产木糖醇[J].生物工程学报,2000,16(3):304-307. 被引量:30
  • 9Cantarella M, Cantarella L, Gallifuoco A, et al. Effect of inhibitors released during steam-explosion treatment of poplar wood on subsequent enzymatic hydrolysis and SSF [J], Biotechnology Progress, 2004, 20 (1):200-206
  • 10原江苏省轻化工业厅纤维水解研究所编.植物纤维水解生产[M].北京:燃料化学工业出版社,1974.

二级参考文献26

共引文献77

同被引文献57

  • 1方祥年,黄炜,夏黎明.半纤维素水解液中抑制物对发酵生产木糖醇的影响[J].浙江大学学报(工学版),2005,39(4):547-551. 被引量:24
  • 2卢雪梅,刘紫鹃,高培基.木质素生物降解的化学反应机制[J].林产化学与工业,1996,16(2):75-82. 被引量:26
  • 3Almeida J R, Modig T, Petersson A, et al. Increased tol- erance and conversion of inhibitors in lignocellulosic hy- drolysates by Saccharomyces cerevisiae [ J ]. Journal of Chemical Technology and Biotechnology, 2007, 82 (4) : 340 - 349.
  • 4Zhang Hou-Rui, Qin Xiang-xiang, Silva S S, et al. Novelisolates for biological detoxification of lignocellulosic hy- drolysate[ J]. Appl Biochem Biotechnol, 2009, 152 (2) : 199 -212.
  • 5Palmqvist E, Hahn-Hagerdal B. Fermentation of lignocel- lulosic hydrolysates. I: inhibition and detoxification [ J -. Bioresource Technology, 2000, 74 ( 1 ) : 17 - 24.
  • 6Casal M, Paiva S, Silva I S, et al. Transport of carboxylic acids in yeasts[J]. FEMS Microbiol Rev, 2008, 32(6) : 974 - 994.
  • 7Roe AJ, Mclaggan D, Davidson I, et al. Perturbation of anion balance during inhibition of growth of Escherichia coli by weak acids [ J -. Journal of Bacteriology 1998,180 (4) : 767 -772.
  • 8Cherrington C A, Hinton M, Chopra I. Effect of short- chain organic acids on macromolecular synthesis in Esche- richia coli [ J]. Journal Applied Microbiology, 1990, 68 (1) :69 -74.
  • 9Zaldivar J, Ingram L O. Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01[J]. Biotechnology and Bioengineering, 1999, 66 (4) : 203 -210.
  • 10Modig T, Lid6n G, Taherzadeh M J. Inhibition effects of furfural on alcohol dehydrogenase, aldehyde dehydrogen- ase and pyruvate dehydrogenase [ J]. Biochem J, 2002, 363 ( 3 ) : 769 - 776.

引证文献3

二级引证文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部