摘要
目的评价自动化细菌鉴定仪Phoenix和Vitek2 Compact以及碳青霉烯酶基因OXA-51（blaOXA-51-like）扩增在不动杆菌属细菌鉴定中的价值。方法收集2006-2007年北京协和医院临床标本中分离的非重复不动杆菌属细菌50株，以扩增性核糖体DNA限制性酶切片段分析（amplified ribosomal DNA restriction analysis，ARDRA）为参考方法，分别采用blaOXA-51-like基因扩增和Phoenix、Vitek2 Compact细菌鉴定仪进行鉴定。结果与ARDRA分子鉴定结果进行对比，blaOXA-51-like基因扩增鉴定方法敏感度为100％，特异度为100％；细菌鉴定仪Phoenix和Vitek2 Compact鉴定到种的准确率分别为44％和56％。结论不动杆菌属仪器表型鉴定准确率不高；对于鲍曼不动杆菌，blaOXA-51-like基因扩增为一种快速可靠的鉴定方法。在研究不动杆菌的耐药机制或同源性分析时，可采用ARDRA方法进行鉴定。
Objective To evaluate the value of automatic bacteria identification systems including Phoenix and Vitek2 Compact, as well as blaOXA-51-like gene amplification in the identification of Acinetobacter species compared with amplified ribosomal DNA restriction analysis （ARDRA）. Methods A total of 50 nonrepeated clinical isolates of Acinetobacter species were collected in PUMCH from 2006 to 2007 and identified by ARDRA, blaOXA-51-like gene amplification, Phoenix and Vitek2 Compact, respectively. Results Compared with ARDRA, the sensitivity and specificity of blaOXA-51-like gene amplification were both 100%. The accuracy rates of Phoenix and Vitek2 Compact were 44% and 56%, respectively. Conclusions The accuracy of phenotypic identification of Acinetobacter species is not ideal, however, blaOXA-51-like gene amplification could be used as a fast and reliable method for identification of A. baumannii. ARDRA is recommended in the study of drug resistant mechanisms and homological analysis of Acinetobacter species.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2009年第1期83-87,共5页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金资助项目（30500023）
