摘要
目的:建立阳离子交换树脂纯化育亨宾总生物碱的工艺。方法:以育亨宾总生物碱溶液为对象,以其主要有效成分育亨宾碱(yohimbine)为指标,考察树脂类型、氨水浓度和乙醇浓度对洗脱效果的影响。结果:阳离子交换树脂D001对育亨宾生物碱成分的交换能力最强,且被树脂吸附的生物碱成分可用氨性乙醇溶液快速洗脱。验证试验结果表明,选择D001树脂,以80%乙醇冲杂后,采用2N氨水80%乙醇作为洗脱液洗脱,总生物碱的含量最高。结论:该工艺操作性强、过程易控制,值得推广应用。
OBJECTIVE To establish a simple method for purifying the total alkaloids from Corynanthe Yohimbe K.schum with ion-exchange resin.METHODS Three cationic ion-exchange resins(D001,001×7 and 001×4)were evaluated for their capacities and the best one was D001.RESULTS The solutions of ethanol with ammonium hydroxide could effectively elute the absorbed alkaloids.The contents of total alkaloids of the extracts obtained using 80% ethanol with(0、1.0 mol and 2.0 mol ammonium hydroxide)as elution solutions were 1.21%,94.32% and 96.24%.CONCLUSION This method is rapid and accurate for the purification of total alkaloids from Corynanthe Yohimbe K.schum.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2009年第2期129-132,共4页
Chinese Journal of Hospital Pharmacy
