摘要
本工作研究了冷消解、微波消解、高压密闭消解方法对超声破碎后细胞中砷的ICP-MS测定的影响,并与直接进样测定结果作对比。结果表明,微波消解法和高压密闭消解法效果较好,测定的精密度以RSD表示,分别为2.1%和1.2%;日间6次测定的精密度分别为1.2%和2.0%;元素加标回收率均在95.7%～108.1%范围。用4种消解方法对应的ICP-MS方法检出限介于0.74～0.93μg/l之间,符合痕量分析要求。本工作还建立了高效液相色谱(HPLC)与电感耦合等离子体质谱(ICP-MS)联用技术对1μmol/L染砷组细胞样品中的砷化学形态进行了初步探讨,发现细胞内除了存在无机砷(As(Ⅲ)和As(Ⅴ))外,同时存在二甲基砷(DMA)和一种甲基砷中间代谢产物。
The effects of direct sampling and three digestion methods were investigated on the determination of arsenic in Chang liver hepatocytes after ultrasonic disintegration were investigated. The results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. The day precision （ present as RSD） of microwave digestion and obturator digestion were 2.1% and 1.2% , the inter-day precision were 1.2% and 2.0%, respectively. The spike recovery for the total As in the sample is 95.7% - 108.1%. The As detection limits with these four sample treatment methods （ including direct sampiing） were 0.74-0.93 p^g/L. In addition, arsenic speciation in Chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. The experimental results indicated that dimethylarsinic acid （DMA） and an intermediate metabolite of DMA were found in Chang liver hepatocytes besides inorganic arsenic （ As （ m ） and As（V））.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2009年第1期19-24,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20675021)资助项目
关键词
细胞消解
高效液相色谱
电感耦合等离子体质谱
肝细胞
砷化学形态
Cell digestion, high performance liquid chromatography-inductively coupled plasma-mass sepctrometry, Chang liver hepatocytes, arsenic speciation
