摘要
以草鱼(Ctenopharyngodon idellus)为实验材料,采用毒性实验方法研究了氯氰菊酯对草鱼肝、鳃、肾组织Na+/K+-ATP酶活性及超显微结构的影响。将草鱼分别浸泡于氯氰菊酯(质量浓度0μg·L-1、1μg·L-1、3μg·L-1、5μg·L-1)溶液中,结果显示,鳃组织Na+/K+-ATP酶活性呈波动性变化;肝、肾组织Na+/K+-ATP酶活性呈抑制效应,肝组织高浓度处理组在72h时出现显著差异(P<0.05),肾组织Na+/K+-ATP酶活性呈抑制效应不显著,用草鱼肝组织Na+/K+-ATP酶活性作为毒理学指标能较好地评价氯氰菊酯毒性效应;超显微观察发现草鱼暴露于氯氰菊酯质量浓度3μg·L-11周后,鳃小叶粘连、鳃上皮细胞损伤脱落、溶酶体数量增加、细胞萎缩及间隙扩大,表明氯氰菊酯暴露对草鱼鳃呼吸膜造成了严重损伤,肝细胞线粒体膨大及部分结构溶解、内质网片断化、细胞内溶酶体增加和出现脂褐素、胞质内出现空泡,表明氯氰菊酯暴露能对草鱼肝的能量代谢及物质合成等生理功能造成影响。
In order to discuss physiological toxicity on carps caused by cypermethrin, the grass carp （Ctenopharyngodon idellus） were exposed to cypermethrin at concentrations of 0 μg· L^-1, 1 μg· L^-1, 3 μg· L^-1, 5 μg· L^-1 from 24 h to 72 h. The results showed that the activity of Na^＋/K^＋-ATP enzyme in gill tissue during different exposure periods varied as waved shape, while the activities of Na^＋/K^＋-ATP enzyme in liver and kidney were inhabited, and the activity of Na^＋/K^＋-ATP enzyme in liver decreased significantly （P〈0.05） in the middle level and high level exposure groups at 72 hour, while the activity of Na^＋/K^＋-ATP enzyme in kidney was inhibited insignificantly（P〉0.05） all the time. The experiment indicates that the liver Na^＋/K^＋-ATP enzyme activity in grass carp could be considered as a valid biomarker for environmental risk assessment. Applying electric microscopic technique, the cytopathology of gill and liver tissue in grass carp exposed to cypermethrinon was observed at concentration of 3μg· L^-1 after a week, and the structure of gill tissue showed that branchial leaflets was damaged; branchial leaflets was connected; epithlia of branchial bow lost, nuclear shrank and lysosome in cell increased. The tissue structure in liver showed that lysosome and lipofuscin increased; glycogen spread and vacuole appeared in cytoplasm. The result indicated that cypermethrin exposure can cause a series of severe toxicity to physiological function in live and gill of grass carp.
出处
《中国水产科学》
CAS
CSCD
北大核心
2009年第1期120-126,共7页
Journal of Fishery Sciences of China
基金
广东省科技厅农业攻关子项目(2005A20105001)
