摘要
目的探讨胃癌高、低发区居民幽门螺杆菌（Ｈｐ）及ｃａｇＡ＋、ｈｓｐＡ＋亚型感染的差异，明确Ｈｐ感染与胃癌的关系。方法用ＥＬＩＳＡ方法分别检测抗Ｈｐ的ＩｇＧ和ＩｇＡ抗体、抗ｃａｇＡ的ＩｇＧ抗体、抗ｈｓｐＡ的ＩｇＧ抗体。结果胃癌高发区山东省临朐县居民Ｈｐ感染率为７３．８％，明显高于低发区苍山县居民感染率（５９．９％）。胃癌高发区居民ＨｐｃａｇＡ＋和ｈｓｐＡ＋亚型感染率分别为５０．９％和１７．０％，亦明显高于低发区居民（分别为３４．０％和１３．２％）。此外，用１３Ｃ－尿素酶呼气试验（１３Ｃ－ＢＵＴ）作标准评价了检测Ｈｐ抗体、ｃａｇＡ＋及ｈｓｐＡ＋方法的灵敏性（分别是９２．３％、６３．２％和２１．９％）和特异性（分别是７１．０％、８０．６％和９５．２％）。Ｈｐ感染，尤其是ｃａｇＡ＋亚型的感染与胃癌高发有明显正相关关系。结论抗Ｈｐ抗体和ｃａｇＡ＋亚型检测方法灵敏度较高，不失为胃癌流行病学研究的良好指标。
Objective To explore the difference in infection of Helicobacter pylori (Hp) and their subtypes cagA + and hspA + in the residents living in the areas with high and low incidence of gastric cancer. Methods Immunoglobulin G and A antibodies against Hp and immunoglobulin G antibodies against cagA + and hspA + were detected for the residents in Linqu and Cangshan Counties of Shandong Province with enzyme linked immunosorbent assay (ELISA). Results Rate of infection with Hp in the residents in Linqu County, an area with high incidence of gastric cancer, was 73.8 percent, significantly higher than that in Cangshan County (59.9%), an area with low incidence. Rates of infection with Hp cagA + and hspA + in the residents in Linqu County were 50.9 and 17.0 percent, respectively, also significantly higher than those in Cangshan County (34.0% and 13.2%, respectively). Sensitivities and specificities of the methods for detecting antibodies against Hp, cagA + and hspA + were 92.3, 63.2 and 21.9 percent and 71.0, 80.6 and 95.2 percent, respectively, evaluated with 13 C urea breath teat ( 13 C UBT). Rate of infection with Hp, especially with Hp cagA +, correlated significantly with the high incidence of gastric cancer. Conclusion The methods for detecting antibodies against Hp and cagA + subtype are more sensitive and can be used as better indicators in epidemiological studies on gastric cancer.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1998年第2期67-69,共3页
Chinese Journal of Preventive Medicine
基金
国家"九五"攻关课题
中美胃癌协作研究基金
