摘要
研究了二氨基绿B（Diamine green B，DB）与牛血清蛋白（BSA）作用的共振光散射（RLS）光谱特征。考察了各种影响因素，优化了实验条件并计算出了BSA与DB的结合比（质量之比）为3．27。结果表明，在优化条件下体系的RLS强度与蛋白质浓度在一定范围内具有良好的线性关系，据此建立了一种蛋白质测定的新方法。在最佳实验条件（DB为2．0×10^-5mol／L，cHCI=7．0×10^-2 mol/L）下，BSA的线性范围是0．03～4．8ug／mL，检出限为1．65ng／mL。该方法已成功用于合成样品及尿液的测定。
The determination of proteins with diamine green B by resonance light scattering （RLS） technique was studied. A new method for the determination of proteins based on the enhancement of RLS was developed with the concentration of hydrochloric acid specified at 7.0 × 10^-2 mol/L. The linear ranges of the calibration graphs were from 0.03 ug/mL to 4.8 ug/mL for bovine serum albumin （BSA）. The corresponding determination limit was 1.65 ng/mL. Samples with foreign substances and urine samples were analyzed satisfactorily.
出处
《化学试剂》
CAS
CSCD
北大核心
2009年第1期35-38,共4页
Chemical Reagents
