摘要
目的:观察凋亡蛋白p53、Bax、Bcl-2,粘附蛋白整合素α3β1、肌营养不良蛋白聚糖(DG)在Heymann肾炎鼠肾小球的表达,以及1,25-(OH)_2D_3对其表达的影响。方法:雌性SD大鼠随机分为对照组和Heymann肾炎模型组;后者经腹腔注射FX1A抗原诱导肾炎模型,再随机分Heymann肾炎组和1,25-(OH)_2D_3组。1,25-(OH)_2D_3组皮下埋置渗透性微量泵,给予1, 25-(OH)_2D_33ng·100g^(-1)·d^(-1),连用4周。检测大鼠尿蛋白、尿足细胞。电镜检测每视野足细胞数、足突平均宽度。免疫组织化学SP法观察肾小球p53、Bax、Bcl-2,整合素α3β1、肌营养不良蛋白聚糖(DG)蛋白的表达。原位末端标记(TUNEL)法检测足细胞凋亡。结果:与对照组相比,Heymann肾炎组大鼠尿蛋白、尿足细胞排泄明显升高;足细胞数目减少,足突宽度增加;肾小球p53、Bax表达增高,Bcl-2表达减少,足细胞凋亡明显增多;整合素α3β1、DG表达明显减少。与Heymann肾炎组相比,1, 25-(OH)_2D_3组尿蛋白、尿足细胞排泄明显减少;肾小球p53、Bax表达减少,Bcl-2表达增加,足细胞凋亡减少;整合素α3β1、DG表达明显增加。结论:1,25-(OH)_2D_3可减少Heymann肾炎鼠肾小球p53、Bax的表达,增加Bcl-2的表达,减少足细胞凋亡。亦可增加整合素α3β1、DG在肾小球中的表达,减少足细胞脱落。从而维持肾小球足细胞数量。
To observe the effect of 1,25-（OH） z D3 on apoptotic protein p53, Bax, Bcl-2 and attachment protein integrin α3β1,dystroglycan（DG） expression in glomeruli in rats with passive Heymann nephritis（PHN）. Method： SD rats were randomly divided into two groups：control group,PHN model group which was induced by antigen（FX1A） peritoneal injection. After these models were successfully established,the）, were randomly divided into two groups：model group without therapy （Heymann group） and 1,25- （ OH ） 2 D3 group with 1,25- （ OH ）2D3 therapy. 1,25- （ OH ） 2 D3 group was embedded with the osmotic minipump for giving 3 ng·100g^-1·d^-1,25- （ OH ） 2 D3. After the treatmemt with 1,25- （ OH ） 2 D3 for 4 weeks, proteinufia, urinary podoctye （ UPC ） were measured. Average numbers of podocytes per field and mean of the foot process width （FPW） were detected under electron microscopy. The expression of p53, Bax, Bcl-2,integrin α3β1, DG in glomeruli was observed by immunohistochemical SP methods. The apoptosis podocyte was detected by the transferase-mediated dUTP nick-end labeling （TUNEL） assay. Result： Compared with control group, the level of urine protein, UPC in Heymann group was significantly increased; average numbers of podocytes per field was dramatically reduced; FPW was increased; the expression of p53,Bax protein increased, Bcl-2 decreased in glomeruli,which increased podocyte apoptosis. Compared with Heymann group,the level of urine protein, UPC in 1,25-（OH）2D3 group was significantly decreased; the numbers of podocytes per field of vision was increased; FPW was reduced; the expression of p53, Bax protein decreased, Bcl-2 increased, which decreased podoctye apoptosis. Conclusion： 1,25-（OH）2D3 could contribute to decrease p53 ,Bax and increase Bcl-2 expression in Hey- mann rats,which reduce podoeyte apoptosis. Furthermore, 1,25-（OH） 2 D3 increased integrin α3β1, DG expression, which prevented podoeyte attachment. So preserve podoeyte numbe including inhibiting apoptosis and detachmen.
出处
《中国药师》
CAS
2009年第1期34-36,共3页
China Pharmacist
