摘要
目的探讨早期诊断糖尿病肾病的方法。方法用酶联免疫吸附试验法检测尿中视黄醇结合蛋白(RBP)和Ⅳ型胶原(Ⅳ-C),免疫发光法检测尿微量清蛋白(mAlb)及转铁蛋白(TF),终点法测N-乙酰-β-D氨基葡萄糖苷酸酶(NAG),Jaffe法测定尿肌酐(Cr)。结果健康对照组中尿TF/Cr为(1.10±0.76)mg/mmol、mAlb/Cr为(1.50±0.81)mg/mmol、NAG/Cr为(5.65±2.40)U/g、RBP/Cr为(2.15±0.93)mg/mmol及Ⅳ-C的含量为(44.68±17.2)ng/mL。在糖尿病无肾病组中,其mAlb的含量与健康对照组相近,差异无统计学意义(P>0.05);而NAG、RBP、TF及Ⅳ-C的含量与健康对照组相比,差异有统计学意义(P<0.05)。在糖尿病的初期肾病组和临床肾病组,mAlb/Cr、NAG/Cr、RBP/Cr、TF/Cr及Ⅳ-C的含量均明显高于健康对照组,差异有统计学意义(P<0.01)。结论联合检测尿NAG、RBP、TF及Ⅳ-C是诊断糖尿病肾病早期损伤灵敏、可靠的实验室指标。
Objective To explore a method for early diagnosis of diabetic nephropathy. Methods ELISA was applied to detecting urine retinol binding protein （RBP） and type Ⅳ collagen （Ⅳ-C）, immunolumineseence assay measuring urinary mieroalbuminn （mAlb） and transferring （TF） ,endpoint method determining urinary N-acetyl-β-D- glucosaminidase （NAG） ,and Jaffe＇s method measuring urinary creatinine （Cr）. Results The levels of urinary TF/ Cr,mAlb/Cr,NAG/Cr,RBP/Cr and Ⅳ-C in healthy control group were （1.10±0.76）mg/mmol,（1.50±0.81）rag/ mmol, （ 5.65 ± 2.40） U/g, （2.15 ± 0.93） rag/retool and （44.68 ± 7.2） ng/mL. In the group of diabetes mellitus without nephropathy,the level of mAlb was approximate to that of healthy control group （P〉0. 05） ,whereas the levels of urinary NAG,RBP,TF and Ⅳ-C were significantly higher （P〈0. 05）. The levels of urinary TF/Cr, mAlb/Cr, NAG/Cr,RBP/Cr and Ⅳ-C in the groups of diabetic nephropathy （initial stage group and clinical nephropathy group） than those in healthy control group （P〈0. 01）. Conclusion Combined detection of NAG,RBP,TF and Ⅳ-c is a sensitive and reliable laboratory indicator of early diagnosis of diabetic nephropathy.
出处
《检验医学与临床》
CAS
2009年第1期19-20,22,共3页
Laboratory Medicine and Clinic
