摘要
采用循环伏安法和交流阻抗法研究了固定在羟基磷灰石薄膜上的DNA与芦丁之间的相互作用。在pH 5.6～7.0范围内,芦丁在DNA修饰电极上的峰电位随pH的增加向负方向移动;在50～800 mV/s扫描速度范围内电极过程同时受扩散和吸附控制,且扩散控制占主导作用;随溶液离子强度增大,芦丁在DNA修饰电极上的表观式量电位不断正移,表明芦丁与DNA之间存在一定的嵌入作用,二者结合形成了超分子化合物。
The interactions of DNA immobilized on the hydroxyapatite （HAp） film with rutin were investigated by means of cyclic voltammetry （CV） and AC impedance. The results show that both the anodic and cathodic peak potentials shift negatively with increasing pH from 5.6 to 7.0. The redox peak currents for rutin at a DNA modified electrode have linear relationship with scan rate from 50 to 800 mV/s, the electrode process is controlled simultaneously by diffusion and adsorption, and the diffusion plays a main role. The apparent potential for rutin at the dsDNA/HAp- PVA/GCE shifts positively with increasing ionic strength of solution, indicating that the intercalation exists between rutin and DNA, leading to formation of supramolecular compounds.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第1期113-116,共4页
Chinese Journal of Analysis Laboratory
基金
泰山学院引进人才(Y05-2-03)项目资助
