摘要
本文应用非程序ＤＮＡ合成（ＵＤＳ）试验，观察了亚硒酸钠（Ｎａ２ＳｅＯ３）和维甲酸（ＲＡ）单独和联合作用对致癌物ＭＮＮＧ诱发的人全血白细胞ＤＮＡ损伤的影响。结果表明，Ｎａ２ＳｅＯ３和ＲＡ均具有抗诱变作用；当Ｎａ２ＳｅＯ３（０．１μｇ／ｍｌ）和ＲＡ（０．１μｇ／ｍｌ）联合作用时，也可明显降低ＭＮＮＧ诱发的ＵＤＳ值，其联合抑制作用大于Ｎａ２ＳｅＯ３的单独作用。实验结果还显示，Ｎａ２ＳｅＯ３作为抗癌剂，它在高剂量时又呈现遗传毒性。
The effects of sodium selenite(Na2SeO3) retinoic acid(RA) and Na2SeO3+RA were examined on MNNG-induced DNA damaged with human wholeblood cells by the test of unscheduled DNA synthesis (UDS) in vitro.The results showed that 0.01～0.05μg/ml Na2SeO3 and 0.02～2.0μg/ml RA significantly suppressed the responses of UDS induced by MNNG.These revealed that Na2SeO3 and RA had the antimutagenic activity.The combined inhibiting effect of (0.1μg/ml) Na2SeO3+(0.1μg/ml) RA on MNNG-induced DNA damage was stronger than the simply effect of Na2SeO3. The results also revealed that Na2SeO3,as inhibitor of mutagenesis,could induce genetoxic effects by itself at higher dose levels.
出处
《癌变·畸变·突变》
CAS
CSCD
1998年第1期15-17,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
亚硒酸钠
维甲酸
抗诱变作用
化学预防
sodium selenite
retinoic acid
antimutagenesis
chemoprevention
