摘要
目的观察应用小分子干扰RNA（siRNA）技术抑制肺癌A549细胞中Survivin表达对细胞凋亡和顺铂耐药性的影响。方法设计、合成特异性抑制Survivin表达的siRNA，转染肺癌A549细胞48h后，检测Survivin基因mRNA表达量以及肺癌细胞凋亡率和对顺铂耐药性变化。结果A549细胞转染Survivin特异siRNA后，Survivin／β-actin基因mRNA表达比例1．17±0．25下降至0．41±0．18，抑制率65．10％；细胞凋亡率由2．67％上升至32．33％；顺铂半数抑制浓度（IC50）由6．37mg／L降低至2．42mg／L。结论通过siRNA特异性抑制肺癌A549细胞中Survivin基因表达可增加凋亡，逆转顺铂耐药。
Objective To evaluate the effect of survivin siRNA silencing survivin gene on the apoptosis rate and sensitivity to eisplafin of lung cancer A549 cells. Methods A549 cells were transfeetcd with survivin siRNA for 48 h. Survivin mRNA expression, apoptosis rate and sensitivity to eisplatin were checked. Results After survivin-siRNA transfection, survivin expression was decreased from 1.17 ± 0.25 to 0.41 ± 0. 18 shown as survivin/β-actin mRNA ratio, and apoptosis rate increased from 2.67% to 32.33% ,and IC50 to eisplatin decreased from 6.37 to 2.42 mg/L. Conclusion Survivin gene silencing by specific siRNA in lung cancer 3.549 cells can induce apoptosis and sensitize cells to cisplafin.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第1期77-78,共2页
Chinese Journal of Experimental Surgery
基金
基金项目：首都医学发展科研基金资助项目（2005-3076）
