摘要
人乳头瘤病毒(HPV)至今没有合适的体外培养系统。为了研究HPV16E7蛋白的免疫学性质,采用分子克隆技术构建了我国地方株(湖北株HB)HPV16E7基因重组表达质粒。经鉴定显示:该基因在大肠杆菌中得到高效表达,分子量约为69KD,为HPV16-HBE7与载体蛋白(β-半乳糖苷酶α肽)融合蛋白。融合蛋白表达量占整个菌体蛋白含量的30%~40%;Westernblot检测结果表明:融合蛋白保留了HPV16E7抗原决定簇的抗原性,能与E7单克隆抗体特异性结合。谈表选系统的建立为进一步研究E7蛋白的免疫学性质提供了充足的材料来源。
A new recombinant expression plasmid of HPV16E7 from a cervical cancer patient was constructed by molecular cloning and named pWHBE7.pWHBE7 was highly expressed in E.coli JM109.The combatant protein MW was 69KD.It was a fusion protein that contains HPV16E7 and vector proteinβgalactose peptide.The HBE7 fusion protein accumulates to 30~40%of total cell protein.The result of Western blot showed this fusion protein retained the antigenicity of HPV16E7 antigenic determinant.It could combime with HPV16E7 McAb.This expression system will supply a sufficient source of E7 protein to facilitate the study of the immunity property of theprotein.
作者
伍欣星
丁晓华
邱小萍
谭云
戴天力
赵文先
Wu Xinxing;Zao Min;Ding Xiaohua;Qiu Xiaoping;Tan Yun;Dai Tianli;Zhao Wenxian(Virus Research Institute,Hubei Medical University,Wuhan 430071)
出处
《免疫学杂志》
CAS
CSCD
北大核心
1998年第1期5-7,共3页
Immunological Journal
基金
湖北省"八五"科技攻关课题基金资助
