摘要
目的:探讨2种玻璃化法对小鼠卵巢组织、器官形态和功能保存作用的影响。方法:以改良的DMEM-F12为玻璃化液,分别采用常规玻璃化法(A组)和超速玻璃化法(B组)冻存小鼠卵巢组织及器官,解冻后通过组织学观察、卵巢组织异体、卵巢器官自体肾被膜下移植,观察动情周期恢复率、恢复时间、卵泡发育状况,并分别以新鲜卵巢组织异体移植、卵巢器官自体移植(C组)为对照,评价2种玻璃化法的冻存效果。结果:①A组、B组、C组卵巢组织异体移植小鼠动情周期出现率为100%,出现动情周期分别10.5±5.4d、8.0±2.2d、6.3±1.0d。A组与C组比,差异显著(P<0.05);B组与C组无差异,A组与B组间也无差异(P均>0.05)。②A组、B组、C组卵巢器官自体移植小鼠动情周期出现率为100%,出现动情周期分别为9.4±0.9d、6.9±1.1d、6.1±1.1d,A组与B、C组相比有统计学差异(P<0.05),而B组与C组间无差异(P>0.05)。移植存活的卵巢组织、器官内均可见不同发育阶段的卵泡,形态正常。结论:2种玻璃化法可有效地冻存卵巢组织及器官,但超速玻璃化法效果较优。
Objective： To explore the effects of the two different vitrification on morphology and function of mouse ovary tissue and ovary organ. Methods： Normal vitrification （group A） and ultra-rapid vitrification （group B） were conducted to preserve mouse ovary tissue and ovary organ with improved DMEM-F12 vitrification solutions. After being thawed, ovary tissues were examined histologically, and allotransplantation or autotrans- plantation under the capsule of the kidney was performed to observe the ratio of recovery of oestrum cycle, the beginning days of recovery of oestrum cycle, follicles development, so as to compare the preserving effects of the two methods. Results： After being thawed and allotransplanted or autotransplanted, mouse ovary tissue or ovary organ kept either in group A or group B all showed a 100% rate in estrous cyclicity recovery, the same applies to fresh transplanted group （group C）. The opening days of estrous cyclicity in mouse ovary tissue and ovary organ of transplanted group were 10.5±5.4 d, 8.0±2.2 d, 6.3±1.0 d and 9.4±0.9 d, 6.9±1.05 d, 6.1±1.1 d, respectively. There was no significant difference between group B and group C in the beginning days of recovery of oestrum cycle after transplanted （P 〉 0.05）, whereas there were significant difference between group A and group C. The differences between the organ transplanted of group A and group B was significant （P〈0.05）. Survival ovarian transplants in each group witnessed the developing follicles at different stages, and the follicles were normal in morphology. Conclusion： The two different vitrifications all effectively preserved mouse ovary tissue or ovary organ, but the ultra-rapid vitrification did it better.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2008年第12期714-719,共6页
Reproduction and Contraception
基金
国家自然科学基金项目,编号:30360112
30760264
宁夏科技公关项目,项目号:2007GG068,2007GG071
