摘要
研究了高山离子芥在低温条件下SA的产生及NO对SA质量分数的影响.结果表明:低温胁迫下,高山离子芥悬浮细胞内源SA质量分数逐渐增强,随着温度的降低,SA质量分数逐渐增强.在0℃处理下,8 h达到最高(2.14μg/g),4℃处理下,24 h达到最高(1.6027μg/g);随着温度的降低,高山离子芥悬浮细胞内NO质量分数明显增强.在NO促进剂SNP、NO合酶(NOS)抑制剂LNNA、NO专一性猝灭剂PTIO及硝酸还原酶(NR)抑制剂NaN_3处理下,研究高山离子芥NO及内源SA质量分数的变化.结果表明:SNP诱发了高山离子芥悬浮细胞内NO及SA的合成积累,LNNA,PTIO抑制了细胞中NO及SA的合成积累,NaN_3对SA影响效果不明显,对NO影响效果显著.每个处理下的高山离子芥在低温条件下的内源SA质量分数均高于常温(20℃)水平,且差异显著(P<0.05).SNP与LNNA,PTIO,NaN_3复合处理中,LNNA与PTIO对SA的抑制作用被消除.
The content change of SA in suspension cells of Chorispora bungeana under low temperature stress and the influence of NO on the content change of SA under low and normal temperatures were tested. The results show that the SA content increased under cold stress with the cold time and cold intension, then dropped to a level higher than that in normal suspension cells. SA level increased to a peak after an 8-hour treatment under 0°C（2.14 μg/g） and increased to a lower peak after 24 hours under 4°C（1.602 7 μg/g） SNP induced the production of SA and NO under different temperatures. Correspondingly, LNNA and PTIO inhibited the production of SA and NO, but the SA content remained relatively stable. Under the treatment of NaN3 the NO content was inhibited by NAN3. The plants under cold stress presented higher levels of SA compared with those under normal temperatures and showed significant differences. Under multiple treatment of SNP, LNNA, PTIO and NAN3, the inhibition of LNNA, PTIO to SA was removed by SNP.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期29-33,共5页
Journal of Lanzhou University(Natural Sciences)
基金
国家自然科学基金(30870378)
国家自然科学基金重大项目(90302010)
国家自然科学基金杰出青年基金(30625008)
甘肃省自然科学基金(3ZS061-A25-063)资助.
关键词
高山离子芥
低温
水杨酸
一氧化氮
Chorispora bungeana
low temperature
salicylic acid
nitric oxide
