摘要
均相体系中,采用H2O2氧化降解法得到低分子量壳聚糖;以其为原料,氯磺酸—二甲基甲酰胺(DMF)为磺化试剂,在较温和的条件下制备低分子量磺化壳聚糖(S-LCTS),产物分子量较磺化壳聚糖(S-CTS)显著降低。红外光谱分析表明,所得产物在1236cm-1和808cm-1处有特征吸收,分别显示出-SO3H基团中S=O和C-O-S的伸缩振动。本文还检测了所得产物对超氧自由基(O2-·)、过氧化氢(H2O2)以及1,1-二苯代苦味酰基自由基(DPPH·)的清除效果。实验结果显示,S-LCTS对O2-·、H2O2和DPPH·的清除效果明显优于S-CTS,显示了良好的抗氧化活性。
Low molecular-weight chitosan was prepared first by oxidative degradation with hydroperoxide（H2O2） under homogeneous conditions. And then, with it as raw material, chlorosulfonic acid （HClSO3）in N, N-dimethylformamide（DMF） as sulfated agent, low molecular-weight sulfated chitosan（S-LCTS） was obtained under mild conditions, and its molecular-weight was considerably lower than normal suffated chitosan （S-CTS）. FTIR spectrum showed that the product had characteristic absorptions at 1236cm^-1 and 808cm^-1, due to sulfo groups, assigned to S = O and C- O- S bond stretchings, respectively. Moreover, the scavenging effects of the product on superoxide radical（ O2^-·）, hydrogen peroxide （ H2O2 ）, and 1,1- diphenyl-2- picrylhydrazyl（ DPPH · ） was determined.The results indicated that the scavenging effects of S-LCTS were much superior to S-CTS＇ and it showed favorable antioxidative activity.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第12期97-100,共4页
Science and Technology of Food Industry
