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CD1d四聚体检测NKT细胞 被引量：2

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摘要 目的:建立一种检测小鼠NKT细胞的方法。方法:首先将α-GalCer负载于空的CD1d四聚体上。将α-GalCer溶于1000mL/L的DMSO中,配成浓度为0.1g/L,之后震荡α-GalCer液,37℃,12h,用之前超声水浴37℃,10min,取12mol/L的上述α-GalCer液加入到1mol/L的四聚体溶液中,将上述二者的混合液37℃,避光,孵育24～48h。然后应用流式细胞术(FCM)检测正常的和腹腔注射α-GalCer5μg3d后的小鼠的脾脏及骨髓中共表达α-GalCer/CD1d四聚体及CD3的NKT细胞。结果:在正常小鼠脾脏及骨髓的淋巴细胞中NKT细胞的比例分别为1.2%和1.6%;腹腔注射α-GalCer5μg3d后小鼠脾脏的淋巴细胞中NKT细胞有轻度的增加,比例为2%和2.7%。结论:CD1d四聚体是检测小鼠NKT细胞的有效工具。
作者 刘景华 窦立萍 王莉莉 于力
机构地区 解放军总医院血液科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2009年第1期82-83,共2页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(30670898)
关键词 CD1d四聚体 NKT细胞 Α-GALCER
分类号 R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献6

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二级参考文献23

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共引文献19

同被引文献24

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  • 8SHARIF S,ARREAZA G A,ZUCKER P. Activation of natural killer T cells by alpha-galactosyl-ceramide treatment prevents the onset and recurrence of autoimmune Type 1 diabetes[J].Nature Medicine,2010,(05):1057-1062.
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二级引证文献5

细胞与分子免疫学杂志
2009年 第1期

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