摘要
24头太湖猪仔猪(大白×二花脸),在出生后即被隔离分为4组,并分别人工哺喂葡萄糖盐水(GS组,n=4)、牛初乳(BC组,n=5)、牛常乳(BM组,n=6)和自然哺乳(PC组,n=9)。前3组在48h后归圈哺乳。经酶联免疫竞争抑制法测定,牛IgG向BC组和BM组仔猪血液中的转移分别在24h和16h左右达到峰值,为(28.19±15.46)g/L和(12.89±1.78)g/L,以后逐渐下降,归圈自然哺乳后7d,两组仔猪血中均仍有5g/L左右牛IgG残留;PC组和GS组未检测到牛IgG免疫反应物。同时观察到,哺喂初乳的PC组和BC组的仔猪腹泻率均低于BM组和GS组。实验猪血涂片白细胞分类计数结果表明,初生时,中性粒细胞为(57.64±7.21)%,淋巴细胞为(40.50±6.25)%。PC组和BC组的中性粒细胞在出生后8h内增加,以后呈下降趋势,48h时分别为(48.12±1.07)%和(54.25±3.45)%;其淋巴细胞在8h时略有下降,后呈上升趋势,48h时分别为(50.17±1.15)%和(42.23±2.75)%。在生后48h内,GS组和BM组的中性粒细胞增加,淋巴细胞下降;48h时的中性粒细胞分别为?
Twenty four newborn Taihu piglets were divided into 4 groups and separately fed by glucose saline (GS, n=4), bovine colostrum (BC, n=5), and bovine milk (BM, n=6) within 48 h after birth, then back to sowfold for suckling freely, the last group naturally suckled all the time (PC, n=9). The serum IgG content was measured by ELISA. The results showed that the transfer of IgG to piglet′s serum reached peak value at 24 h for BC (28.19±15.64) g/L and 16 h for BM (12.89±1.78) g/L respectively, then declined. About 5 g/L of IgG in serum of both BC and BM piglets remained on day 9. The percentage of piglets diarrhea in PC and BC were lower than that in BM and GS. At birth, the average of leukocytes differentiation count were neutrophiles (57.64±7.21)%, lymphocyte (40.50±6.25)%. In PC and BC, the neutrophiles increased within first 8 h, then slightly declined to (48.12±1.07)% at 48 h and the lymphocytes increased to (50.17±1.15)% at 48 h. In GS and BM the neutrophiles increased and lymphocytes decreased within 48 h. Monocytes, eosinophiles and basophiles accounted for the least count with less variation in all groups.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第1期32-35,共4页
Chinese Journal of Veterinary Science
基金
国家教委博士点专项基金
关键词
初乳
转移
仔猪
免疫
colostrum
IgG
transfer
piglets