摘要
β-葡聚糖是植物细胞壁中结构性非淀粉多糖,存在于各种饲料农作物中[1]。作为一种抗营养因子,β-葡聚糖使食糜具有很高的粘度,阻碍肠道消化液与食糜的混合,影响营养物质特别是蛋白质和脂肪的消化吸收,降低饲料的利用率。地衣芽孢杆菌分泌的β-葡聚糖酶可分解β-葡聚糖[2]。在青贮发酵过程中,这种酶可降解大麦β-葡聚糖,有效提高家畜、家禽对饲料的利用率[3]。从地衣芽孢杆菌WS-6中克隆到β-1,3-1,4-葡聚糖酶基因并进行测序,将该基因与大肠杆菌表达载体pET32a进行连接,转化大肠杆菌BL21,使该基因得到了有效的表达,为进一步在食品级宿主菌中的表达奠定了基础。
The barley glucan is an anti-nutritional factor that hinders the digestion of barley-based diets.β-glucanase that is secreted from Bacillus species,can hydrolyze glucan and improve digestion of barley-based diets effectively.Therefore,it has been applied in animal feed additive industry.The research and application of β-glucanase as a silage additive have been being developped quickly.In this research,the β-1,3-1,4-glucanase gene from Bacillus licheniformis WS-6 was cloned and sequenced,then ligated into pET32a and transformed in Escherichia coli BL21.Eventually,the effective heterogeneous expression was accomplished.This work has laid the foundation for achieving a heterogeneous expression in lactis acid bacteria for further study.
出处
《生物技术通报》
CAS
CSCD
2008年第6期135-138,共4页
Biotechnology Bulletin
基金
国家973计划“秸秆资源生态高值化关键过程的基础研究”(2004CB719704)
国家农业科技成果转化资金项目“青贮饲料微生物添加剂技术成果转化”(2006GB2G400336)
新疆自治区高技术研究与发展计划项目“微生物青黄贮菌剂的分子改良及应用”(200511107)
