摘要
通过反复洗涤与低速离心（１０００×ｇ，１０ｍｉｎ），从大豆下胚轴得到的细胞壁碎片具有ＡＴＰ酶活力．酶活依赖于Ｍｇ２＋的存在，受Ｋ＋刺激和钒酸钠的强烈抑制．经加ＫＩ的悬浮液洗涤，碎片ＡＴＰ酶受叠氮化钠抑制很少，几乎不受硝酸盐抑制．表明所得的ＡＴＰ酶基本上为质膜ＡＴＰ酶．本方法简化了质膜的制备过程，在质膜及质膜ＡＴＰ酶的生理研究中有较高的应用价值．
Cell wall fractions were prepared from Glycine max hypocotyls by low speed centrifugation (1 000×g, 10 min) of homogenate and repeated washing. The fractions contained ATPase activity that is dependent on Mg2+, stimulated by KCl and strongly inhibited by vanadate. Some inhibition of the activity was observed by azide but this inhibition was removed after washing with KI. And little inhibition was observed by KNO3. The results suggest that the ATPase activity associated with the cell wall fractions is predominantly of plasma membrane origin. Thus this simple method provides a quick and easy means of studying plasma membrane ATPase activity.
关键词
质膜
质膜ATP酶
大豆
plasma membrane
PMATPase
Glycine max L.
