摘要
目的：探讨血管紧张素-（1-7）[Ang-（1-7）1对血管紧张素Ⅱ（AngⅡ）诱导大鼠系膜细胞（GMC）增殖及血管紧张素Ⅱ受体对血管紧张素-（1-7）作用的影响。方法：选择对数生长期GMC，分为对照组、AngⅡ组、Ang-（1-7）组、[Sar^1,Ile^8]-AngⅡ＋Ang-（1-7）组、、PD123319＋Ang-（1-7）组，通过[^3H]-Thymidine及[^3H]-Leucine掺入分别测定GMC的DNA、蛋白质合成；结晶紫染色检测细胞数日，观察系膜细胞增殖情况，探讨Ang-（1-7）是否通过AngⅡ受体发挥作用。结果：Ang-（1—7）可抑制AngⅡ诱导GMC的DNA、蛋白质合成及细胞数日增加；[Sar^1,Ile^8]-AngⅡ和PD123319不影响Ang-（1—7）的上述作用。结论：Ang-（1—7）能抑制基础和AngⅡ诱导的GMC增殖，其作用的发挥不通过AngⅡ的AT1和AT2受体介导。
Objective： To explore the effect of angiotcnsin-（1-7）[Ang-（1-7）]on proliferation in cultured raftS Glomerular Mesangial Cels （GMC）induced by Angiotensin Ⅱ （Ang Ⅱ） and angiotensin Ⅱ receptor and angiotensin-（1-7）. Methods： GMC in logarithmic growth phase were incubated , and then were divided into control ,Ang Ⅱ,Ang-（1-7）,[Sar^1,Ile^8]-Ang Ⅱ ＋Ang-（1-7）、、PD123319＋Ang-（1-7） groups. The synthesis of DNA and protein measured bv Incorporation of [^3H]-thymidine and [^3H]-leueine;cell numbers of rates GMC were detected by crystal violet.Results：Ang-（1-7）inhibited synthesis of DNA and protein of cultured rat＇s GMC induced by Ang Ⅱ.Ang-（1-7）also reduced the numbers of cultured rat＇s GMC induced by Ang Ⅱ.The efects of Ang-（1-7）could not be blocked by both [Sat6,IleS]-Ang 1I ,a specific Ang 11 ATj receptor antagonist;and Ang Ⅱ AT2 receptor antagonist PD123319.Conelusion：Ang-（1-7）can inhibit proliferation of cultured rat＇s GMC induced by Ang Ⅱ,the efects of Ang-（1-7） can not be inhibited by Ang Ⅱ AT1 and AT2 receptor antagonist.
出处
《中国当代医药》
2008年第24期21-22,43,共3页
China Modern Medicine
