摘要
目的体外培养获得神经干细胞并初步研究重组人肝细胞生长因子(human recombinant hepatocyte growth factor,HGF)对神经干细胞(neutal steal cells,NSCs)分化能力的影响。方法从胚胎大鼠脑组织中分离得到神经干细胞,并行免疫荧光鉴定。检测不同浓度的HGF(5ng/ml、10ng/ml、20ng/ml、40ng/ml)对神经干细胞分化的影响。结果体外培养的神经干细胞球巢蛋白(nestin)表达阳性,由神经干细胞球分化的细胞神经元特异性烯醇化酶(neuron specific enolase,NSE)和胶质纤维酸性蛋白(galial fibrillary acidic protein,GFAP)表达阳性。并且不同浓度HGF作用下的神经元细胞分化率不同。结论HGF对NSCs分化及较长时间存活具有重要作用,20ng/ml的HGF可以促进较好的分化效果。
Objective To obtain neural steam cell by invitro culture and explore the effect of HGF on neural steal cell differentiation. Methods The cells from the embryonic mouse brain tissue were primary cultured and indentified by immunofluorescence stain. The NSCs was affected by 5,10,20,40ng/ml HGF then used to explore the influence on NSCs differentiation. Results Neural spheres express nestin and the cells differentiated from the spheres express NSE or glial fibrillary acidic protein（GFAP）. HGF in different concentration have different influence on NSCs differentiation rate. Conclusion HGF has important effects on NSCs differentiation, And 20ng/ ml HGF would induce NSCs differentiate into neuron in higher rate.
出处
《立体定向和功能性神经外科杂志》
2008年第6期351-354,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery
关键词
肝细胞生长因子
胎鼠
神经干细胞
分化
Hepatocyte growth factor
Embryonic mouse
Neural steal cell
Differentiation
