摘要
为探讨ＩＬ－６基因转导对人乳腺癌细胞体内外生长特性的影响，我们构建了含人ＩＬ－６ｃＤＮＡ的逆转录病毒载体ｐＬＩＬ６ＳＮ。采用脂质体介导的方法将ｐＬＩＬ６ＳＮ导入ＭＣＦ－７细胞中，对基因转导细胞的体外生长、粘附特性及裸鼠体内的实验性转移能力进行了测定。实验结果表明，经ＩＬ－６基因修饰的ＭＣＦ－７细胞虽显示了明显的体外生长抑制，裸鼠体内实验性转移能力却获得增强。
Objective: To investigate the influence of IL6 gene transduction on human mammary carcinoma cell growth both in vitro and in vivo.Method: retroviral vector pLIL6SN which contained human IL6 cDNA was constructed and transferred into MCF7 cell line with the mediation of lipofection.IL6 gene transfectant growth and adhesion potential in vitro was assayed and its metastatic competence in nude mice was examined. The results showed that the growth of IL6 genetransferred MCF7 cell was markedly inhibited in vitro,and that the metastatic potential in vivo was enhanced.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1998年第1期41-43,共3页
Chinese Journal of Clinical Oncology
基金
国家博士后科学基金
