摘要
采用免疫组织化学ＡＢＣ法检测６６例经１０％福尔马林固定，石蜡包埋的大肠癌手术切除癌组织中的嗜铬蛋白Ａ（ＣｈｒｏｍｏｇｒａｎｉｎＡ，ＣＧＡ）表达及ｐ５３蛋白聚集，并定量计数ｐ５３免疫阳性细胞，进一步分析ＣＧＡ与ｐ５３蛋白聚集变化的关系，结果显示：大肠癌ｐ５３ＣＧＡ阳性率分别为６１％（４０／６６）和３８％（２５／６６）；ＣＧＡ阳性及阴性病例ＣＧＡｐ５３阳性率分别为８０％（２０／２５）和４９％（２０／４１），ＣＧＡ阳性肿瘤ｐ５３阳性率明显高于ＣＧＡ阴性肿瘤（χ２＝６．３４，Ｐ＜０．０２５）；ｐ５３、ＣＧＡ均阳性的大肠癌２０例ｐ５３阳性细胞数为８５７±７０６／ｍｍ２，ｐ５３阳性、ＣＧＡ阴性表达者２０例ｐ５３阳性细胞数５７１±４７８／ｍｍ２，前者高于后者（ｔ＝２．３３，Ｐ＜０．０５）。结论：ｐ５３蛋白聚集与内分泌样癌细胞的分化呈现较明显的一致性。
Purpose: p53 protein accumulation and endocrinelike tumor cells and their correlation in colonic cancer. Method: chromogranin (CGA) and p53 protein were determined on 66 cases with the cancer by immunohistochemical (ABC) method.Quantitative analysis of p53 protein accumulation by recording the number of p53 immunostaining positive cells was performed to correlate the changes of CGA and p53. Result: The percentage of p53 and CGA positive immunoactivity in colonic cancer tissues were 61% (40/66)and 38% (25/66)respectively.p53 protein accumulation occurred more frequently in patients with CGApositive colonic cancer (80%) than in those with CGAnegative cancer (49%). The difference of p53 accumulation between these two groups was significant (P<0.025, χ2 test). The number of p53 immunostaining positive cells in the cases with or without CGApositive cells were 857±706/mm2 and 571±478/mm2 respectively, and a significant difference was observed(P<0.05). Conclusion: The results suggest that there is a strong concordance between p53 protein accumulation and differentiation of endocrinelike tumor cells in colonic cancer. The alteration of p53 suppressing gene may be one of the important molecular changes to affect the differentiation of endocrinelike tumor cells in human colonic cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1998年第1期16-18,共3页
Chinese Journal of Clinical Oncology
