摘要
目的:探讨应用荧光定量聚合酶链反应(FQ-PCR)检测乙型肝炎病毒脱氧核糖核酸(HBV-DNA)在宫内感染监测中的意义。方法:应用FQ-PCR检测142例HBsAg阳性孕妇血清及其新生儿脐带血的HBV-DNA,应用酶联免疫吸附试验(ELISA)检测孕妇血清及其新生儿脐带血的乙肝血清学标志物,并对其结果进行分析。结果:新生儿脐带血HBV-DNA阳性率(16.2)高于HBsAg的阳性率(9.85),P<0.01;血清HBeAg阳性孕妇,其新生儿脐带血HBV-DNA阳性率为41.0(16/39),高于HBeAg阴性孕妇组的阳性率6.80(7/103),P<0.01;新生儿脐带血的HBV-DNA阳性率随孕妇HBV-DNA含量增加而增加(P<0.01)。结论:孕妇血清HBV-DNA高含量是乙型肝炎宫内感染的高危因素,应用FQ-PCR检测新生儿脐带血HBV-DNA是监测乙型肝炎发生宫内感染的较敏感指标。
Objective：To discuss the significance of the detection of hepatitis B virus deoxyribonucleic acid（HBV-DNA） using fluorescence quantitative PCR（FQ-PCR） in intrauterine infection monitoring.Methods：HBV-DNA was quantitatively examined by FQ-PCR in 142 pregnant women with positive HBsAg and their newborns,and serologic markers of HBV were detected by enzyme linked immunoadsorbent assay（ELISA）.All data of detection were analyzed.Results：The positive rate of cord blood HBV-DNA in infants（16.2%） was significantly higher than the positive rate of HBsAg（9.85%）（P〈0.01）.The positive rate of cord blood HBV-DNA in newborns with HBeAg-positive mother was 41.0%（16/39）,which was markedly higher than that of HBeAg-negative mother（6.80%,7/103）（P〈0.01）.And the positive rates of cord blood HBV-DNA in infants increased with the rising contents of serum HBV-DNA in pregnant women（P〈0.01）.Conclusion：The high content of serum HBV-DNA in pregnant women is one of the high risk factors of HBV intrauterine infection.And the detection of cord blood HBV-DNA in newborns using FQ-PCR is a sensitive index for monitoring the HBV intrauterine infection.
出处
《中国计划生育学杂志》
北大核心
2008年第12期747-748,共2页
Chinese Journal of Family Planning
关键词
乙型肝炎病毒
宫内感染
荧光定量
PCR
Hepatitis B virus
Intrauterine infection
Fluorescence quantitative
PCR
