摘要
花粉发芽实验ＮＪ８９—１雄性不育度高达９９．２５％以上且稳定，人工授粉试验ＮＪ８９—１的雌性育性正常，说明ＮＪ８９—１是一个雄性不育雌性可育突变体。不育株自然授粉后代中育性分离结果表明ＮＪ８９—１雄性不育性受单隐性核基因控制。等位性测验表明ＮＪ８９—１不育基因与ｍｓ１～ｍｓ５均不等位。ＮＪ８９—１在败育时期、减数分裂、四分体形成、小孢子壁发生、花药壁发育、花粉粒形态等诸多方面与ｍｓ１～ｍｓ６突变体均存在差异，而与ｓｔ２～ｓｔ５突变体却有相似的减数分裂变异如联会异常等，但是ＮＪ８９—１的雌性育性正常，ｓｔ２～ｓｔ５突变体的雌性均高度不育。因而ＮＪ８９—１是一个既不同于ｍｓ类型又不同于ｓｔ类型的新雄性不育突变体。建议将ＮＪ８９—１雄性不育基因符号定为ｍｓ７。
Artificial pollination experiments revealed that the female fertility of NJ89-1 was normal, then, NJ89-1 was a male-sterile female-fertile mutant. Pollen germination experiments showed that the male sterility of NJ89-1 was pretty high and stable with a pollen germination percentage less than 0.75%. Genetic data led to the conclusion that the male sterility of NJ89-1 was controlled by a single recessive nuclear gene. Allelism tests indicated that the male sterile gene of NJ89-1 was non-allelic to the male sterile genes of ms1, ms2, ms3, ms4 and ms5. The cytomorphological observations on anther and pollen development showed that NJ89-1 was different from ms1￣ms6 mutants in many aspects such as abortion stage, meiosis, tetrad formation, pollen wall and anther wall etc. NJ89-1 displayed similar meiotic abnormalities of asynapsis or desynapsis to those of st2￣st5 mutants, but differed from st2￣st5 mutants in female fertility, namely, the female of NJ89-1 was normal, while those of st2￣st5 mutants were strongly impaired. Therefore, NJ89-1 was a new male sterile mutant. The male sterile gene symbol of NJ89-1 was proposed to be ms7.
出处
《大豆科学》
CAS
CSCD
北大核心
1998年第1期32-38,共7页
Soybean Science
基金
国家自然科学基金
"八五"攻关资助
关键词
大豆
雄性不育
突变体
遗传学
细胞学
Soybean
Male Sterility
Mutant
Inheritance
Cytomorphology
