摘要
将90只30日龄的岭南黄鸡肉鸡(公母各半),随机分为3组:对照组(30只)、内毒素组(30只)、氨基胍治疗组(30只)。分别在试验后1、3、5、7、9 h每组各宰杀6只提取血清和肝组织。检测血清和肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性。结果显示,内毒素血症时血液和肝组织中丙二醛的含量升高,SOD与GSH-Px的活力降低,而在氨基胍治疗组情况明显改善。结果表明,肉鸡内毒素血症时存在着明显的脂质过氧化作用,而氨基胍可以明显的减轻内毒素诱导的自由基诱导的脂质过氧化损伤。
Ninety broilers were randomly divided into three groups. Group Ⅰ （n = 30） was the normal control. Group Ⅱ were intravenously injected with lipopolysaccharides （from Salmonella typhimurium）. Group Ⅲ received certain dose of lipopolysaccharides from salmonella after injected aminoguanidine. The serum MDA contents, T- SOD,GSH-Px activities and the MDA contents,T-SOD,GSH-Px activities of the liver tissue were examined at different times. The results showed serum and liver tissue MDA contents of group Ⅱ evidently （P〈0.05） increased and activities of serum and liver tissue T-SOD, the serum and liver tissue GSH-Px activities evidently（P〈0.05） decreased after endotoxin injection. The experimental results indicated that lipid perioxidation induced by endotoxin played a certain role in endotoxemia of broilers and the aminoguanidine could reduce the injury and effectively protect the cell functions and structure.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2008年第12期1423-1425,1429,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30750574
30371068)
关键词
肉鸡
内毒素血症
氨基胍
脂质过氧化
broilers
endotoxemia
aminoguanidine
lipid perioxidation
