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表达大鼠脑源性神经营养因子腺相关病毒(rAAV-BDNF)对体外培养海马神经元保护作用的研究 被引量：4

Protective effects of adeno-associated viral gene transfer of BDNF on the hippocampal neurons
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摘要 目的:研究表达大鼠脑源性神经营养因子腺相关病毒(rAAV-BDNF)对体外培养海马神经元的保护机制。方法:通过去血清培养诱导神经元凋亡后转染rAAV-BDNF,通过DAPI、PI及Actin染色来统计rAAV-BDNF对血清饥饿引起的细胞凋亡的数量及形态的影响。结果:rAAV-BDNF病毒能够有效地抑制血清饥饿引起的细胞凋亡,其保护效率在65左右,并可以有效地保护血清饥饿引起的细胞形态损伤。结论:rAAV-BDNF可通过抑制凋亡的途径保护体外培养的海马神经元。 Objective：To investigate the effective mechanism for transfection of rat brain derived neurotrophic factor（BDNF） gene by recombinant adeno-associated virus in the cultured hippocampal neurons.Methods：rAAV-BDNF was injected into the serum free-induced neurons.DAPI,PI and Actin stain was performed to measure the apoptosis cells and morphogenesis of the cells.Results：After the gene transfer,the survival rate of the serum free-induced neurons was increased by 65%.Conclusion：The recombinant adeno-associated virus vector can protect the serum free-induced neurons from Apoptosis.
作者 张惊宇 赵节绪
机构地区 吉林大学第一医院神经内科
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2008年第12期1100-1102,共3页 Chinese Journal of Immunology
关键词 rAAV-BDNF 细胞凋亡 基因治疗 rAAV-BDNF Apoptosis Gene therapy
分类号 R-332 [医药卫生] R392 [医药卫生—免疫学]
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参考文献7

  • 1Body J G, Gordon T. Glial cell lined-derived neurotrophic factor and brain-derived neurotrophic factor sustain the axonal regeneration of chronically axotomized motoneuronsinvivo [ J ]. Exp Neurol, 2003 ; 183 ( 2 ) : 610- 619.
  • 2金国华,田美玲,秦建兵,黄镇,徐慧君,钟世镇.BDNF、NGF对体外长期培养的胚基底前脑胆碱能神经元的影响[J].神经解剖学杂志,2001,17(4):337-341. 被引量：12
  • 3Schabitz W R, Berger C, Koolmar R et al. Effect of brain-derived neurotrophic factor treatment and forced arm use on functional motorrecovery aner small cortical ischemia[ J ]. Stroke, 2004; 35 (4) : 992-997.
  • 4张惊宇,赵节绪,陈嘉峰,王宇虹,杨永梅.表达大鼠脑源性神经营养因子(BDNF)腺相关病毒的构建及体外表达分析[J].脑与神经疾病杂志,2007,15(1):25-29. 被引量：4
  • 5Schabitz W R, Sommer C, Zoder W et al. Intravenous brain-derived neurotrophic factor reduces infarct size and counter-regulates Bax and Bcl-2 expression after temporary focal cerebral isehemia [ J ]. Stroke, 2000; 31 (9) :2212-2217.
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二级参考文献13

  • 1Schabitz WR,Berger C,K01lmar R,et a1.Effect of brain-deriVed neurotrophic factor treatment and forced arm use on functional motorrecovery aner small cortical ischemia,Stroke.2004,35(4):992～997
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  • 9Boyd JG Gordon T Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor sustain the axonal regeneration of chronically axotomized motoneuronsinvivo.EXp Neur01.2003,183(2):610～619
  • 10金国华,徐慧君,武义鸣,田美玲,钟世镇.BDNF、NGF对体外培养的胚胆碱能神经元生长发育的影响[J].神经解剖学杂志,1997,13(3):230-234. 被引量：10

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二级引证文献9

中国免疫学杂志
2008年 第12期

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