摘要
以播种后得到的日本厚朴幼苗为试材对日本厚朴的组织培养技术进行了研究,采用正交试验比较了不同外植体和激素配比对愈伤组织诱导的影响,光培养和暗培养两种培养方式以及不同激素种类和配比对增殖和生根培养的影响。试验结果表明,日本厚朴组织培养的最佳外植体是顶芽,最佳培养方式为暗培养,最佳启动培养基为B5+2,4-D4.0mg/L+NAA1.0mg/L,最佳增殖培养基为B5+6-BA2.0mg/L+NAA1.0mg/L。
This paper studies the tissue culture of Magnolia obovata taking the seedlings of Magnolia obovata obtained after planting as as experimental material, and by using the orthogonal test, compares the influences of different explants and hormone mixture ratios on callus induction, and the influences of two kinds of culture modes, light culture and dark culture, and different kinds and mixture ratios of hormone on the proliferation and rootage culture. The results indicate that the best explant of Magnolia obovata is terminal bud, the best culture mode is dark culture, the best starting medium is B5＋2,4-D4.0 mg/L＋NAA 1.0 mg/L, and the best proliferation culture medium is B5＋6-BA2.0mg/L＋NAA 1.0mg/L.
出处
《科技情报开发与经济》
2008年第33期114-115,共2页
Sci-Tech Information Development & Economy
关键词
日本厚朴
组织培养
光培养
愈伤组织诱导
Magnolia obovata
tissue culture
light culture
callus induction
