摘要
用常规心肌细胞内微电极技术探讨了5-HT对豚鼠右心室乳头状肌AP的影响,5-HT 1～30μM表现剂量相关地延长APD_(20)、APD_(50)、APD_(90)和ERP,但对ERP/APD_(90)值,RP、APA和Vmax均无明显影响。 赛庚啶8μM明显缩短APD和ERP,并拮抗5-HT对APD的影响。在含赛庚啶的灌流液中,5-HT只有在30～100μM时显示延长APD的作用。以上结果说明5-HT对乳头状肌AP的影响可能是通过与5-HT受体作用所致。
The effect of 5-hydroxytrypamine (5-HT) on the action potential (AP) of guinea pig papillary muscles were studied by means of intracellu-lar microelectrodes. Concentrations of 5-HT from 1 to 30 μM had the effect of prolonging the action potential duration (APD20, APD60> APD80) and the effective refractory period (ERP) , in a concentration-dependent manner. However, ratio of ERP to APD80 (ERP/APD80) , resting potential (RP) , action potential amplitude (APA) and maximum rate of rise of action potential (Vmax) were not obviously affected by 5-HT.Cyproheptadine of 8 μM shortened both APD and ERP and antagonized the effect of prolonging APD of 5-HT. When preparation bathed in perfusion fluid containing 8 μM cyproheptadine, the effect of prolonging APD of 5-HT were showed for only 30 and 100 μM. The results indicate that the effect of 5-HT on AP of papillary muscles may were induced by its effect on 5-HT receptor.
出处
《基础医学与临床》
CSCD
1990年第2期92-95,共4页
Basic and Clinical Medicine
关键词
5-羟色胺
赛庚啶
乳头状肌
AP
5 -hydroxytryptamine cyproheptadine papillary muscles action potentials
