摘要
目的目前临床广泛使用的涂层人工血管由于使用具有细胞毒性的交联剂如乙醛和甲醛,植人体内可带来一些副作用从而影响人工血管的愈合。为减少这些不良后果的发生,作者进行了热交联胶原涂层的机织涤纶人工血管的实验研究。材料和方法将牛胶原蛋白浸渍涂层涤纶人工血管内外壁并使用热交联固定,通过血管壁渗水率,形态学以及力学性能等体外试验评价热交联胶原蛋白涂层人工血管是否达到人工血管植入前标准。结果结果显示未涂层人工血管管壁渗水率在经过胶原蛋白涂层和热交联后降低了99%,达到了植入时不漏血的目的。胶原蛋白涂层占重(297±23 mg/g)较InterGardTN血管的涂层重量(313±25 mg/g)稍低一些。结论本研究初步证实热交联胶原蛋白涂层人工血管可操控性好,渗水率低。同时在体外具备良好的生物降解性能。
Objective It has been reported that coated vascular prostheses commonly used in vascular surgery had side effects due to toxicity of the crosslinkers such as glutaraldehyde and formaldehyde. To diminish undesirable side effects of chemical cross-linking of biodegradable materials, the authors designed a woven polyester vascular prosthesis coated with collagen by thermally crosslinking （the WCT-graft ）. Materials and method In this study, a porous woven polyester （Dacron） graft was coated with collagen suspension by thermally crosslinking. Then, it was investigated in vitro to evaluate its potential for use as a new vascular graft impervious to blood. For in vitro investigation, the water permeability, coating weight, morphology, and mechanical properties of the WCT-graft were compared to those of control and commercially available collagen-coated （ InterVasenlar ） grafts. Results The water permeability of the controls （381.4 mL · cm^-2 · min^-1 at 120 mm Hg） was reduced 〉99% by collagen impregnation, rendering the graft im pervious to blood. The coating weight of the collagen was 297 ± 23 mg/g of graft, producing a slightly lower value than that of the InterGardTN prosthesis （313 ±25 mg/g）. Conclusion This study demonstrated that the use of a biodegradable collagen as biological sealant and crosslinked thermally can be a feasible approach to prepare impervious textile arterial prostheses.
出处
《中国分子心脏病学杂志》
CAS
2008年第6期357-361,共5页
Molecular Cardiology of China
关键词
胶原蛋白
人工血管
热交联
渗水率
Vascular graft
Collagen
thermally Crosslinking
Water permeability
