摘要
本实验观察了海马内多次注射神经生长因子（NGF）对小鼠学习记忆的影响，包括剂量－效应关系和时间－效应夫系以及3H－Leu掺入突触体蛋白质的示踪研究。结果表明：（l）与注射人工脑脊液（ACSF）的对照组相比，在五种剂量（0．01,0．05，0．l，0．5，1．0pg／pl，双侧海马内注射，每次1．0pl，共注射4次，累积剂量为0．04，0．2，0．4，2．0，4．0pg）中除0．01pg／外，其他4种剂量的NGF均能显著或极显著地提高小鼠Y一迷宫分辨学习的24h记忆保持率，其中0．05pg／pl剂量的效果最佳；（2）最佳剂量的NGF易化记忆的效应在训练后24h最好，96h出现明显的减退（P＜0.05）；（3）最佳剂量的NGF能使行为训练后小鼠脑突触体蛋白质的3H－Leu掺入量增加。以上结果说明，适当剂量的NGF在一定的时间内具有增强记忆的功效，并能促进脑内突触体蛋白质的合成。
The innuences of NGF by direct intrahippocampal injechon on discriminatioo learning andmemory in Y-maze and the incorporation of 3H--Leu into synaptosomal proteins were observed in young mice.The resultS are as follows (l) Ivhcn NGF of different doses (0.05, 0.l, 0.5, l.0 μg/ μl, the cumulative doses were0.2, 0.4, 2.0, 4.0 μg respectively) were applied, the memory consolidation was improved signincantly as comparedwith the ACSF control (P< 0.01). Among them the 0.05 μg/ al group was most effective. NGF at 0.01 μg/ alhad no significant effect. The faciliating effect of this dose at 24 hours began to decrease significantly (P< 0.05)at 96 hours after training. The NGF at 0.05μg/ al could enhance the incorporation of 3H-Leu intosynaptosomal proteins of the cerebral cortex very significantly (P< 0.01). The results suggest6d that the suitsbledose of NGF could improve the memory consolidation and the syuaptosomal protein synthesis in the brain ofmicee.
出处
《神经科学》
SCIE
CAS
1997年第1期22-26,共5页
Chinese Journal of Neuroscience
关键词
神经生长因子
海马
注射
分辨学习
nerve growth factor
intrahippocampal injection
Y-maze
discrimination learning
incorporation of ~3 H-Leu
synaptosomes
