摘要
用高效液相－电化学检测器和旋转计数方法检测6－羟多巴胺（6－hydroxydopamine，6－OHDA）损毁大鼠纹状体多巴胺（dopamine，DA)排空与旋转的关系。D1/D2混合性激动剂阿扑吗啡（apomorphine，APO，0．2mg/kg，iP）可使33只大鼠中的20只出现旋转．其中15只旋转强度＞5次／分钟，平均为12．l±5．8次／分钟。D1选择性激动剂SKF38393、D2选择性激动剂LY171555有类似APO的作用。然而，左旋千金藤啶碱（（-）－stepholidine,SPD)仅使14只大鼠出现旋转，其中6只旋转＞5次/分钟，平均为6．41±1．3次/分钟。33只损毁大鼠健测纹状体DA合量平均为9．9±1．1μg/g湿组织，损侧为2．8±2．9μg/g湿组织。旋转＞5次／分钟的大鼠，其健侧和损侧纹状体内DA含量分别为10．1±0．8和0．3±0．1μg/g湿组织。说明纹状体内DA排空程度与旋转行为有密切关系。当损侧纹状体排空小于51％时．APO不能引起任何旋转，而SPD的阈值为88．2％。APO、SKF38393、LY171555和SPD引起＞5次／分钟的旋转，分别需要损侧纹状体内DA排空达96±4％，94±4％，95±4％和98±2％以上。
To elucidate to what extent of DA depletion that SPD showed its agonist, the correlation between therotation ac striatal DA depletion in the 6 - OHDA - lesioned rats was estimated by virtue of HLC- ECD andcounting of circling. Out of the total 33 rats sampled, 20 rats were evoked to rotate contralaterally by the D1/D2mixed agonit APO(0. 2 mg/kg, ip).Among them, 15/20 rats circled more than 5 turns/min, in the average of12. 1 ± 5. 8 turns/min. The D1 selective agonist SKF38393, and the D2 selective agonist LY171555 had the similareffects as APO. However, SPD challenged14 rats to rotate and only 6 rats showed the circling intensity of more than5 turns/min and averaged6. 4 ± 1. 3 turns/min. The mean DA contents in the intact and the lesioned striatacalculated from 33 lesioned rats were 9.9±1. 1 and 2.8 ± 2. 9μg/g wet tissue, rspectively. In the rats who rotated morethan 5 turns/min, the DA contents for intact and lesioned striata were 10. 1± 0. 8 and 0. 3 ± 0. 1 μg/g wet tissue,repectively. The intimate cirrelation between the rotation and the depletion of striatal DA content were observed. Asfar as the DA in the lesioned striatum depleted lees than 51 %, APO failed to produce any rotation. For SPD, thethreshold was 88 %. The extent of DA depletion for induction of rotation more than 5 turns/min for APO,SKF38393, LY171555 and SPD should be more than 96±4 %, 94±4 %, 95± 4 % and 98±2 %,respectively.
出处
《神经科学》
1997年第3期103-109,共7页
Chinese Journal of Neuroscience
关键词
千金藤啶碱
多巴胺
旋转行为
震颤麻痹
stepholidine
dopamine
rotation behavior
high performance liquid chromatography-electrochemical detector (HPLC-ECD)
6 -hydroxydopamine
