摘要
背景与目的:Ezrin蛋白是胞浆内细胞膜与细胞骨架的连接蛋白,参与调节细胞粘附、运动,可能与多种恶性肿瘤的侵袭进展有关。而Ezrin与胰腺癌侵袭转移的关系及其机制尚无明确报道。本研究旨在探讨Ezrin基因与胰腺癌侵袭转移的关系。方法:采用逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)方法半定量检测32例新鲜胰腺癌组织、19例胰外转移灶组织、10例非肿瘤胰组织中Ezrin mRNA的表达。结果:32例胰腺癌组织中Ezrin mRNA的表达较非肿瘤胰组织高,其光密度比值分别为1.9±1.1和0.9±0.5,两者之间的差异具有统计学意义(P<0.05);胰外转移灶组织Ezrin mRNA的表达为2.6±0.8,高于癌组织中的表达水平,两者之间的差异具有统计学意义(P<0.05)。有胰外浸润的癌组织Ezrin mRNA的表达为2.1±1.2,与无胰外浸润的癌组织的1.4±0.7相比,两者之间的差异具有统计学意义(P<0.05)。将32例胰腺癌按不同的临床病理特征分组分别比较Ezrin mRNA的表达,结果显示肿瘤分化差、远处转移的病例中Ezrin mRNA表达更高。结论:Ezrin基因的表达与胰腺癌的侵袭转移密切相关,有可能作为预测胰腺癌转移的一个标志物。
BACKGROUND ＆ OBJECTIVE. Ezrin protein is a membrane cytoskeletal crosslinker between the cell membrane and cytoskeleton. Increasing evidence has shown that Ezrin may be associated with tumor invasion and progression. This study was to explore the correlation of Ezrin to metastasis of pancreatic carcinoma. METHODS： The expression of Ezrin mRNA in fresh specimens of 32 pancreatic carcinoma, 19 metastatic lesions and 10 non-cancerous pancreatic tissues were assessed by reverse transcription-polymerase chain （RT-PCR）. RESULTS： The mRNA expression of Ezrin was significantly higher in pancreatic carcinoma tissues （1.9±1.1） than in non-cancerous tissues （0.9±0.5）（P〈0.05）, significantly higher in metastatic lesions （2.6±0.8） than in the primary tumor focus （1.9±1.1） （P〈0.05）, and significantly higher in pancreatic carcinoma lesions with metastasis （2.1±1.2） than in those without （1.4±0.7） （P〈0.05）. Ezrin mRNA was differently expressed in tumors with different clinical pathologic characters. It was highly expressed in poorly differentiated or distantly metastatic carcinomas. CONCLUSIONS. Ezrin gene is closely related to invasion and metastasis of pancreatic carcinoma, which might be used as a marker in predicting the metastasis of pancreatic carcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2008年第12期1337-1340,共4页
Chinese Journal of Cancer
