期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

拟南芥DELLA下游的GASA基因表达研究 被引量：8

原文传递
导出
摘要 赤霉素(gibberellins,GAs)信号转导途径相关组分的分离与功能研究对于最终阐明GA作用机制十分重要,目前赤霉素信号途径关键因子DELLA的下游组分研究较少.拟南芥中受GA调控的GASA(GA-Stimulated in Arabidopsis)家族共有15个基因,预测所有GASA蛋白的N端均有一可剪切的信号肽,C端含有12个半胱氨酸的GASA保守结构域.RT-PCR结果证实,在DELLA突变体gai-t6和rga-24以及二者双突变体中,GASA4和GASA6的表达上调而GASA1和GASA9下调,与GASA4和GASA6表达受外源赤霉酸(GA3)促进而GASA1和GASA9表达受GA3抑制的结果相一致.除此之外,其他一些GASA基因表达也分别受GA3和脱落酸(ABA)的独立或共同调控.大部分GASA基因在根、茎、叶、花和幼嫩荚果中均有表达.利用启动子驱动GUS报告基因的方法研究了GASA6,GASA7,GASA8,GASA9,GASA10,GASA11和GASA12等7个基因的器官表达特异性,发现在生长分化旺盛的组织器官及离层区均有这些基因的表达,可能与细胞的分裂和生长有关.本研究为GASA基因家族在GA和ABA信号途径中的功能研究提供重要依据。
作者 张盛春 王小菁
机构地区 华南师范大学生命科学学院 广东省植物发育生物工程重点实验室
出处 《科学通报》 EI CAS CSCD 北大核心 2008年第22期2760-2767,共8页 Chinese Science Bulletin
基金 国家自然科学基金(批准号:30570165) 广东省科技攻关(批准号:2005B20901014和2006A20101007)资助项目
关键词 拟南芥 GASA DELLA 赤霉素 脱落酸
分类号 Q943.2 [生物学—植物学]
  • 相关文献

参考文献26

  • 1Fleet C M, Sun T P. A DELLAcate balance: The role of gibberellin in plant morphogenesis. Curr Opin Plant Biol, 2005, 8(1): 77-85
  • 2Ueguchi-Tanaka M, Ashikari M, Nakajima M, et al. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 2005, 437(7059): 693-698
  • 3Peng J, Carol P, Richards D E, et al. The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Genes Dev, 1997, 11(23): 3194-3205
  • 4Silverstone A L, Mak P Y, Martinez E C, et al. The new RGA locus encodes a negative regulator of gibberellin response in Arabidopsis thaliana. Genetics, 1997, 146(3): 1087-1099
  • 5Gan Y, Kumimoto R, Liu C, et al. GLABROUS INFLORESCENCE STEMS modulates the regulation by gibberellins of epidermal differentiation and shoot maturation in Arabidopsis. Plant Cell, 2006, 18(6): 1383-1395
  • 6Yu H, Ito T, Zhao Y X, et al. Floral homeotic genes are targets of gibberellin signaling in flower development. Proc Natl Acad Sci USA, 2004, 101(20): 7827-7832
  • 7Achard P, Herr A, Baulcombe D C, et al. Modulation of floral development by a gibberellin-regulated microRNA. Development, 2004, 131(14): 3357-3365
  • 8Shi L, Gast R T, Gopalraj M, et al. Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J, 1992, 2(2): 153-159
  • 9Taylor B H, Scheuring C F. A molecular marker for lateral root initiation: The RSI-1 gene of tomato (Lycopersicon esculentum Mill) is activated in early lateral root primordia. Mol Gen Genet, 1994, 243(2): 148-157
  • 10Ben-Nissan G, Lee J Y, Borohov A, et al. GIP, a Petunia hybrida GA-induced cysteine-rich protein: A possible role in shoot elongation and transition to flowering. Plant J, 2004, 37(2): 229-238

二级参考文献31

  • 1Keller B. Structural cell wall proteins. Plant Physiol, 1993, 101 (4): 1127-1130
  • 2Carpita N C, Gibeaut D M. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J, 1993, 3(1): 1-30
  • 3McQueen-Mason S J, Cosgrove D J. Expansin mode of action on cell walls (Analysis of wall hydrolysis, stress relaxation, and binding). Plant Physiol, 1995, 107(1): 87-100
  • 4Wu Y, Cosgrove D J. Adaptation of roots to low water potentials by changes in cell wall extensibility and cell wall proteins. J Exp Bot, 2000, 51(350): 1543-1553
  • 5Somerville C, Bauer S, Brininstool G, et al. Toward a systems approach to understanding plant cell walls. Science, 2004, 306(5705): 2206-2211
  • 6Goodwin W, Pallas J A, Jenkins G I. Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus. Plant Mol Biol, 1996, 31(4): 771-781
  • 7Gyorgyey J, Nemeth K, Magyar Z, et al. Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells. Plant Mol Biol, 1997, 34(4): 593-602
  • 8Han Y, Gomez-Vasquez R, Reilly K, et al. Hydroxyproline-rich glycoproteins expressed during stress responses in cassava. Euphytica, 2001, 120(1): 59-70
  • 9Otte O, Barz W. Characterization and oxidative in vitro cross-linking of an extensin-like protein and a proline-rich protein purified from chickpea cell walls. Phytochemistry, 2000, 53(1): 1-5
  • 10Bradley D J, Kjellbom P, Lamb C J. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell, 1992, 70(1): 21-30

共引文献2

同被引文献79

  • 1张淑江,李菲,章时蕃,张慧,孙日飞.“十一五”我国大白菜遗传育种研究进展[J].中国蔬菜,2011(6):1-8. 被引量：18
  • 2WU WeiGuang 1,2,3,HUANG JiKun 1,3 & DENG XiangZheng 1,3 1 Institute of Geographic Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing 100101,China,2 Department of Economics and Management,Zhejiang Forestry University,Linan 311300,China,3 Center for Chinese Agricultural Policy,Chinese Academy of Sciences,Beijing 100101,China.Potential land for plantation of Jatropha curcas as feedstocks for biodiesel in China[J].Science China Earth Sciences,2010,53(1):120-127. 被引量：8
  • 3曾虹燕,方芳,苏杰龙,李昌珠,蒋丽娟.麻疯树籽油提取技术[J].江苏农业学报,2005,21(1):69-70. 被引量：18
  • 4张春晓,王文棋,蒋湘宁,陈雪梅.植物基因启动子研究进展[J].Acta Genetica Sinica,2004,31(12):1455-1464. 被引量：87
  • 5黄志刚,李玲,陈兆平,文方德.SPINDLY与赤霉素的信号转导[J].植物学通报,2005,22(1):100-106. 被引量：10
  • 6郭承刚,王朝文,李建富,苟平,王代鹏,陈兰云,武逵,袁理春.麻疯树物候期和花的发育动态观察[J].现代农业科技,2007(1):12-13. 被引量：20
  • 7Ogawa M, Kusano T, Koizumi N, et al. Gibberellin-responsive genes: high level of transcript accumulation in leaf sheath meristematie tissue from Zea mays L[J]. Plant Molecular Biology, 1999, 40(4): 645-657.
  • 8Shi L, Gast R T, Galparaj M, et al. Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato[J]. The Plant Journal, 1992, 2(2): 153-159.
  • 9Herzog M, Dorne A M, Grellet F. GASA, a gibberellin-regulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene[J]. Plant Molecular Biology, 1995, 27: 743-752.
  • 10Ben-Nissan G, Weiss D. The petunia homologue of the tomato gastl: transcript accumulation coincides with gibberellin-induced cell elongation[J]. Plant Molecular Biology, 1996, 32: 1067-1074.

引证文献8

二级引证文献24

科学通报
2008年 第22期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部