摘要
目的观察雾化吸入和静脉应用盐酸戊乙奎醚对油酸诱导大鼠急性肺损伤(ALI)的影响。方法采用静脉注射油酸复制ALI大鼠模型。将40只SD大鼠随机均分为四组:空白对照组(Ⅰ组)、ALI组(Ⅱ组)、盐酸戊乙奎醚雾化吸入组(Ⅲ组)和盐酸戊乙奎醚静脉注射组(Ⅳ组)。每隔60分钟记录大鼠BP、肺动脉压(PAP),并检验动脉及混合静脉血气。注射油酸240min后检测支气管肺泡灌洗液(BALF)蛋白定量、肺髓过氧化物酶(MPO)活性。结果Ⅲ、Ⅳ组较之Ⅱ组能够缓解由注射油酸引起的PAP升高和PaO2/FiO2下降,Ⅲ组缓解更为明显(P<0.05)。Ⅲ、Ⅳ组BALF蛋白定量、白细胞计数和肺MPO活性均比Ⅱ组降低,Ⅲ组降低更为明显(P<0.05)。结论盐酸戊乙奎醚雾化吸入较静脉注射更能缓解油酸引起的ALI。
Objective To investigate the therapeutic effect of penehyclidine in rats with oleic acld-induced acute lung injury（ALI）. Methods Forty male SD rats were randomized equally into four groups of normal control （group I ）, ALI （group Ⅱ ）, penehyclidine inhalation （group Ⅲ）, and intravenous penehyclidine （group Ⅳ）. Blood pressure（ BP）, pulmonary artery pressure（ PAP）, arterial and mixed venous blood gas analysis were performed every 60 minutes during the experiment. The neutrophils and total protein （TP）content in bronchoalveolar lavage fluid （BALF）, lung myeloperoxidase（MPO） activity were determined after the experiments. Results PAP and PaO2/ FiO2 were improved in group Ⅲ. and Ⅳ compared with those in group I and Ⅱ. TP,neutrophil aecout and differentiation in BALF and lung MPO were lower in group Ⅲ and Ⅳ than those in group Ⅱ. Conclusion Penehyelidine inhalation is better than intravenous penehyelidine in attenuating ALI induced by oleic acid in rats.
出处
《临床麻醉学杂志》
CAS
CSCD
2008年第11期964-966,共3页
Journal of Clinical Anesthesiology
基金
上海市科委科研计划项目(编号O55407030)
