摘要
目的探讨Nogo-B蛋白在新生大鼠脑白质损害(WMD)时的表达变化及意义。方法将2日龄SD大鼠(n=90),随机分为实验组和对照组各45只,制成WMD模型;采用TUNEL法测定神经细胞凋亡及免疫组化(SP)法检测Nogo-B蛋白在脑室周围白质区不同时间点的表达变化。结果实验组神经细胞凋亡在低氧缺血后3d达到高峰。与对照组比较,在4、12、24h、3、7d有统计学意义(P<0.05)。实验组Nogo-B蛋白在低氧缺血后4h表达增加,3d达高峰,在4h、12h、3d、7d的表达与对照组相比,差异有统计学意义(P<0.05)。结论新生大鼠脑白质损害时,Nogo-B蛋白可能参与诱导脑白质神经细胞凋亡。
Objective To study the expression of Nogo-B protein in Neonatal rat with white matter damage. Methods Ninety 2-day postnatal SD rats were randomly divided into experimental group （ n = 45 ） and control group （ n = 45 ）, then the Medcl White matter damage was set up;the dynamic changes of expression of Nogo-B protein were studied by using technical and Apeptotie cells were detected in theses tissued by TUNEL. Results The strong positive cell count of TUNEL in experimental group was upregulated and peaked at 3d after WMD,dcmonstrating significant differences at 4h,12h,24h,3d and 7d compared with that observed in the control group（ P 〈 0.05 ）. The expression of Nogo-B protein in periventricular white matter and corpus callosum in WMD groups were up-regulated at 12h and peaked at 3d after HI, demonstrating significant differences at 12h, 24h ,3d and 7d compared with that observed in the shamsurgery group（ P 〈 0.05） .Condusion In the WMD neonatal rot, .Nogo-B protein may related to induce apeptosis of neurocytes.
出处
《四川医学》
CAS
2008年第11期1475-1477,共3页
Sichuan Medical Journal
