摘要
目的探讨OX40L基因变异与早发急性冠状动脉综合征风险之间的关系。方法采用聚合酶链反应-序列特异性扩增(PCR-SSP)技术检测187例年龄≤55岁、早发急性冠状动脉综合征(ACS)患者和138例冠状动脉造影正常者rs3850641位点A/G变异的基因型、等位基因及其分布频率;采用冠状动脉造影测量病变血管支数(DVN)和狭窄程度积分(SSI);应用ELISA法测试血浆sOX40L、血管细胞黏附分子(VCAM-1);应用散射比浊法测试血浆C反应蛋白(CRP)水平;分析OX40L基因变异与ACS风险、冠状动脉病变程度和血浆3个细胞因子之间的关系。结果OX40L基因A/G变异与早发ACS风险显著关联,G等位基因比野生型早发ACS风险增高2.031倍(OR=2.03),变异型比野生型DVN、SSI、血浆OX40L、VCAM-1和CRP显著增高。结论OX40L基因变异可能与早发ACS风险和冠状动脉病变程度有关;OX40L过度表达、细胞黏附和炎症反应可能是ACS发病的分子生物学基础。
Objective To investigate the relationship between genetic variation of OX40L and susceptibility risk of premature acute coronary syndrome （PACS）. Methods Sequence specific primers-polymerase chain reaction （PCRSSP）was used for the detection of genotypes and alleles of rs3850641 A/G loci in OX40L gene in 187 PACS cases （≤55 years）and 138 controls. Diseased vessel numbers （DVN）and stenostic severity integral （SSI） by coronary arteriogram and serum levels of sOX40L, C-reactive protein （CRP） and vascular cell adhesion molecule-1 （VCAM-1） by enzyme -linked immunosorbent assay （ELISA） and nephelometry were measured. The relationship between OX40L gene variants and PACS risk, SSI as well as plasma levels of three cytokines were analysed. Results OX40L gene variant was significantly associated with PACS risk. G allele had a significantly higher risk for PACS than the wildtype （OR=2.03）. Variant genotype had significantly higher levels of DVN, SSI, sOX40L, CRP and VCAM-1 than the wildtype. Conclusions OX40L genetic variation may be associated with PACS risk and extent of coronary lesion. OX40L overexpressing, cellular adhesion and inflammatory reaction may be molecular biological mechanism for susceptibility of PACS.
出处
《北京医学》
CAS
2008年第12期710-713,共4页
Beijing Medical Journal
基金
镇江市科技局社会发展基金资助项目(SH2006048)
