摘要
目的:观察不同微环境下经静脉注入抗生素在椎间隙内的浓度分布,为椎间隙感染的抗生素治疗提供依据。方法:通过椎间隙内注射金黄色葡萄球菌,建立狗椎间隙感染模型,经下腔静脉缓慢推注头孢唑啉液(40mg/kg),30min后,获取少许感染椎间盘,彻底清除病灶。饲养1周后,再次下腔静脉推注头孢唑啉液(40mg/kg),30min后,取椎间隙感染病灶清除后的椎间隙内组织及正常椎间盘髓核。高效液相色谱法检测不同微环境下椎间隙内组织药物浓度。结果:正常椎间盘髓核、椎间盘感染、椎间隙感染病灶清除后的椎间隙组织内药物浓度分别为(4.62±1.74)μg/mg、(7.00±2.48)μg/mg和(14.11±2.10)μg/mg,椎间隙感染病灶清除后的椎间隙组织内药物浓度较正常椎间盘髓核和感染椎间盘内药物浓度有显著升高(P<0.01)。结论:椎间隙感染病灶清除后,椎间隙内头孢唑啉浓度有明显提高。
Objective：To determine the antibiotic concentration in disc space under different micro-circumstances and to provide theoretic basis for clinical antibiotic treatment of pyogenic discitis.Methods：The pyogenic discitis model was established by injecting Staphylococcus aureus into the intervertebral space of the dog.Cefazolin was injected into the inferior vena cava,and 30 minutes later,the infected disc（B）was obtained and the infected disc debrided.One week later,cefazolin 40 mg/kg was injected and the tissues of infected disc space was harvested after debridement（D）and normal nucleus（E）.The cefazolin concentrations of B,D,and E were tested by High-pressure Liquid Chromatography method.Results：The cefazolin concentrations of the normal nucleus,infected disc and the tissues of the infected disc space after debridement were（4.62±1.74）μg/mg,（7.00±2.48）μg/mg,and（14.11±2.10）μg/mg,respectively.Significant difference in cefazolin concentration existed between the tissues of infected disc space after debridement and the normal nucleus,as well as between the tissues of infected disc space after debridement and the infected disc（P〈0.01）.Conclusions：The concentration of cefazolin in the tissues of infected disc space rises significantly after debridement.
出处
《蚌埠医学院学报》
CAS
2008年第6期747-749,共3页
Journal of Bengbu Medical College
基金
安徽省卫生厅自然科学研究资助项目(2002B053)
关键词
椎间隙感染
头孢唑啉
高效液相色谱
狗
pyogenic discitis
cefazolin
high-pressure liquid chromatography
dog
