摘要
从焦化废水活性污泥中分离出1株能利用喹啉作为唯一碳、氮源及能源的高效降解菌,16S rRNA鉴定为Pseudomonassp..降解试验表明,菌株能将192--911 mg/L的喹啉在3--8 h内有效地降解,去除率均在96%--98%.最适降解温度为30℃,最适降解初始pH值为8.0.跟踪分析降解中间产物发现：在整个降解过程中,至少有43%的喹啉转化为2-羟基喹啉,其后有0.69%的2-羟基喹啉转化为2,8-二羟基喹啉,随后再转化为8-羟基香豆素;另外,至少有48%的喹啉中氮直接转化为氨氮,且外加碳源能促进氨氮的进一步转化,控制好碳氮比将能有效去除喹啉及其转化的各种污染物.
A bacterial strain BW003, which could utilize quinoline as sole carbon, nitrogen and energy source, was isolated from the activated sludge in a coking wastewater treatment plant. According to the 16S rRNA gene sequence analysis, the strain was identified as Pseudomonas sp.. Biodegradation experiments showed that the strain could degrade 192-911 mg/L of quinoline efficiently within 3-8 h, and the removal rates of quinoline were ranged from 96% to 98 %. The optimum conditions for the degradation were 30℃ and pH 8. Metabolic products analysis revealed that at least 43 % quinoline was first transformed into 2-hydroxyquinoline,0. 69 % 2-hydroxyquinoline was then transformed into 2,8- dihydroxyquinoline,and subsequently into 8-hydroxycoumarin in the process of biodegradation. Additionally, at least 48% of nitrogen in quinoline was transformed into NH3-N directly and external carbon source could promote the nitrogen transformation, demonstrating that the quinoline and its metabolic products could be eliminated if controlling proper C/N ratio.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2008年第12期3546-3553,共8页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2004AA649090
2006AA06Z336)
