摘要
目的研究丰富环境对局灶性脑梗死后大鼠微血管新生的影响。方法采用开颅电凝法制作SD大鼠右侧大脑中动脉缺血(MCAO)模型,术后24 h随机分为丰富环境组(EE组)和标准环境组(SE组)。另设假手术组(Sham组)。免疫组织化学法检测大鼠颅脑血管内皮生长因子(VEGF)、Ⅷ因子表达,测定微血管密度。结果EE组和SE组大鼠大脑中动脉栓塞后,缺血区神经元变性、坏死,术后1 dVEGF在缺血周边区开始表达,3 d达高峰,EE组大鼠经干预后后期VEGF表达和微血管数目高于SE组大鼠。结论丰富环境可促进脑梗死大鼠VEGF、Ⅷ因子表达,促进微血管新生,有利于脑损伤修复。
Objective To evaluate the effect of enriched environment on vascular endothelial growth factor （VEGF） expression and angiogenesis in rats after unilateral local cerebral infarction. Methods The right middle cerebral artery occlusion（MCAO） was performed with electric coagulation in SD rats, then the models were randomly divided into enriched environment stimulation group（ EE group） and standard environment stim- ulation group（SE group）. The sham-operation rats were as control group. The expression of VEGF and factor Ⅷ, and angiogenesis in the boundary zone of brain infarction were measured at 1, 3, 7, 14, 28 d after operation. Results After MCAO operation, degeneration and necrosis of neurons in the infarction zone were found in the rats of EE group and SE group. VEGF started expressing in the boundary zone of the infarction at 1 d after operation, and reached peak level at 3 d after operation. Later, VEGF expression and microvessel density （MVD） of EE group were significantly higher than those of SE group. Conclusion Enriched environment stimulation can increase the expression of VEGF and promote the blood vessel regeneration in rats after unilateral local cerebral infarction.
出处
《国际内科学杂志》
CAS
2008年第11期629-631,642,F0003,共5页
International Journal of Internal Medicine
关键词
脑缺血
血管内皮生长因子
毛细血管新生
丰富环境
Cerebral ischemia
Enriched environment
Vascular endothelial growth factor
Angiogenesis
