摘要
目的研究含不同Ｃａ２＋浓度的Ｓｔ．Ｔｈｏｍａｓ停搏液对未成熟心肌的保护作用。方法采用２０℃低温９０ｍｉｎ缺血再灌注幼兔离体心脏灌注模型。结果再灌注１０ｍｉｎ，高Ｃａ２＋组ＬＶＤＰ、±ｄｐ／ｄｔ恢复率明显高于低Ｃａ２＋及中Ｃａ２＋组（Ｐ＜０．０５，Ｐ＜０．０１）。但再灌注２０ｍｉｎ后，高Ｃａ２＋组左室功能呈下降趋势，再灌注末，与其它组间无显著差异，与之相比，再灌注期间，低Ｃａ２＋及中Ｃａ２＋组左室功能则呈渐进性恢复。结论再灌注后，高Ｃａ２＋组心肌细胞外间隙滞留的Ｃａ２＋进入细胞内，加速了Ｃａ２＋诱导的ＡＴＰ耗能过程，高Ｃａ２＋浓度的Ｓｔ．Ｔｈｏｍａｓ停搏液对未成熟心肌不能提供满意的保护作用。
Objective To determine the myocardial protective effect of St.Thomas cardioplegia at different calcium concentrations on immature myocardium.Methods Isolated perfused neonatal rabbit hearts were subjected to 20℃ hypothermic 90 minutes of global ischemia in Langendorff mode of operation.Results Although the recovery of LVDP ±dp/dt max at calcium content of 2.4 mmol/L was significantly higher than that at 0.6 and 1.2 mmol/L calcium (P<0.05,P<0.01,respectively) after 10 minutes of reperfusion, the hemodynamic recovery was found declining in the next 20 minutes. By the end of 30-min reperfusion, the functional recovery at 2.4 mmol/L calcium did not differ from that at 0.6 and 1.2 mmol/L calcium. Conversely, postischemic LV function at 0.6 and 1.2 mmol/L calcium got improved gradually during the 30 minutes of reperfusion.Conclusion Calcium accumulated in the extracellular space during ischemia enters myocardial cell after reperfusion, and accelerates the ATP consumption induced by calcium, which would be possibly responsible for the results noted in this study. It is concluded that St.Thomas cardioplegia at high calcium concentration can not provide immature myocardium with optimal myocardial protection.
出处
《徐州医学院学报》
CAS
1997年第3期251-254,I000,共5页
Acta Academiae Medicinae Xuzhou
基金
江苏省教委自然科学基金
关键词
晶体停搏液
钙
心肌保护
未成熟心肌
Crystalloid cardioplegia Calcium Myocardial protection Immature myocardium
